Tomonari K, Lovering E
Transplantation Biology Section, MRC Clinical Research Centre, Harrow, Middlesex, UK.
Immunogenetics. 1988;28(6):445-51. doi: 10.1007/BF00355377.
Two monoclonal antibodies specific for the mouse T-cell receptor (Tcr) have been established by immunization with a V beta 11+ T-cell clone, clone C6. One is a rat antibody, KT11 (IgG2b, k), specific for the V beta chain of C6, V beta 11. This was demonstrated by the fact that the strain distribution pattern of KT11+ cells was similar to that of V beta 5, 8, 9, 11, 12, and 13 and that the gene that encodes the molecule detected by KT11 was closely linked to V beta 8 in (B10 X SJL)F1 X SJL backcross mice. Furthermore, V beta of C6 has been cloned from a lambda gt10 cDNA library and was demonstrated to be identical to the V beta 11 published sequences. All strains of mice that do not express major histocompatibility complex class II E molecules had higher numbers of KT11+ cells than E+ strains. The KT11+ population in A strain mice and its H-2 congenic strains, however, was not affected by the presence or absence of E molecules. The other is a mouse antibody, KTL2 (IgM), specific for the idiotope of the Tcr expressed on the clone C6. Both antibodies were mitogenic and induced cytotoxicity. Expression of epitopes detected by KT11 or KTL2 was down-modulated by a T3 epsilon-specific antibody 145-2C11.
通过用Vβ11 + T细胞克隆C6进行免疫，已建立了两种对小鼠T细胞受体（Tcr）特异的单克隆抗体。一种是大鼠抗体KT11（IgG2b，κ），对C6的Vβ链Vβ11特异。这一点可通过以下事实证明：KT11 +细胞的品系分布模式与Vβ5、8、9、11、12和13的相似，并且在（B10×SJL）F1×SJL回交小鼠中，编码KT11所检测分子的基因与Vβ8紧密连锁。此外，已从λgt10 cDNA文库中克隆出C6的Vβ，并证明其与已发表的Vβ11序列相同。所有不表达主要组织相容性复合体II类E分子的小鼠品系，其KT11 +细胞数量均高于E +品系。然而，A品系小鼠及其H - 2同基因品系中的KT11 +群体不受E分子存在与否的影响。另一种是小鼠抗体KTL2（IgM），对克隆C6上表达的Tcr独特型特异。两种抗体均有丝分裂原性并诱导细胞毒性。KT11或KTL2所检测表位的表达可被T3ε特异抗体145 - 2C11下调。
