Okano H, Endo T, Shiokawa S, Kyoden Y, Ishibashi Y, Kono A, Nishi K, Fukumaki Y
Kyushu Cancer Center Research Institute, Fukuoka.
J Biochem. 1988 Aug;104(2):162-4. doi: 10.1093/oxfordjournals.jbchem.a122433.
We present evidence for the continuous erythroid differentiation of a cell line, KU-812-F, without the addition of an inducer. Erythroid differentiation of these cells was confirmed according to the following findings: 1) erythroid morphology; 2) existence of glycophorin A and carbonic anhydrase I as a membrane marker protein and an enzyme characteristic of erythroid cells, respectively; 3) synthesis of adult type (HbA and HbA2) and fetal type (HbF) hemoglobins, as detected on isoelectric focusing; and 4) transcription of the alpha-, beta-, and gamma-globin genes, as detected on Northern blot analysis.
我们提供了在不添加诱导剂的情况下，KU-812-F细胞系持续进行红系分化的证据。根据以下发现证实了这些细胞的红系分化：1）红系形态；2）分别存在作为膜标记蛋白的血型糖蛋白A和作为红系细胞特征性酶的碳酸酐酶I；3）在等电聚焦上检测到成人型（HbA和HbA2）和胎儿型（HbF）血红蛋白的合成；4）在Northern印迹分析中检测到α-、β-和γ-珠蛋白基因的转录。
