Bonduelle M L, Dodd R, Liebaers I, Van Steirteghem A, Williamson R, Akhurst R
Department of Medical Genetics, Vrije Universiteit, Brussels, Belgium.
Hum Reprod. 1988 Oct;3(7):909-14. doi: 10.1093/oxfordjournals.humrep.a136808.
Human chorionic gonadotrophin beta (HCG-beta) is a trophoblast marker. Its expression is normally limited to syncytiotrophoblast cells of chorionic villi, although it is known to be secreted from the human embryo as early as 7 days post-fertilization. To examine the onset of embryonic transcriptional activity of the gene encoding this polypeptide we have performed in-situ hybridization to cellular RNAs of human tripronucleate preimplantation embryos. We see expression of HCG-beta RNA at the 6-8-cell stage, before morphological differentiation between trophectoderm and inner cell mass is apparent. We believe that this RNA is the product of de novo transcription from the embryonic genome, since transcripts are only observed in embryos of at least 2 days post-fertilization in age.
人绒毛膜促性腺激素β（HCG-β）是一种滋养层标志物。其表达通常局限于绒毛膜绒毛的合体滋养层细胞，尽管早在受精后7天就已知它会从人类胚胎中分泌出来。为了研究编码该多肽的基因的胚胎转录活性的起始，我们对人类三原核植入前胚胎的细胞RNA进行了原位杂交。我们在6-8细胞阶段观察到HCG-β RNA的表达，此时滋养外胚层和内细胞团之间的形态分化尚不明显。我们认为这种RNA是胚胎基因组从头转录的产物，因为转录本仅在受精后至少2天的胚胎中观察到。
