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过表达的pp60c-src中自磷酸化位点的调控

Regulation by the autophosphorylation site in overexpressed pp60c-src.

作者信息

Kmiecik T E, Johnson P J, Shalloway D

机构信息

Department of Molecular and Cell Biology, Pennsylvania State University, University Park, 16802.

出版信息

Mol Cell Biol. 1988 Oct;8(10):4541-6. doi: 10.1128/mcb.8.10.4541-4546.1988.

DOI:10.1128/mcb.8.10.4541-4546.1988
PMID:2460746
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC365532/
Abstract

We show that overexpressed pp60c-src is phosphorylated at Tyr-416 and has increased specific kinase activity when isolated from cells incubated with vanadate, a tyrosine phosphatase inhibitor. This supports the hypothesis that transient Tyr-416 phosphorylation modulates the activity of overexpressed pp60c-src in vivo. Mutagenesis indicates that Tyr-416 modulates pp60v-src activity as well.

摘要

我们发现，过表达的pp60c-src在酪氨酸416位点发生磷酸化，并且当从与酪氨酸磷酸酶抑制剂钒酸盐孵育的细胞中分离出来时，其比激酶活性增加。这支持了这样一种假说，即酪氨酸416位点的瞬时磷酸化在体内调节过表达的pp60c-src的活性。诱变表明，酪氨酸416位点也调节pp60v-src的活性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/48e1/365532/e8c16da981fd/molcellb00070-0582-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/48e1/365532/306aa0dc3ca7/molcellb00070-0580-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/48e1/365532/fe6d5b51e4cb/molcellb00070-0581-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/48e1/365532/e8c16da981fd/molcellb00070-0582-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/48e1/365532/306aa0dc3ca7/molcellb00070-0580-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/48e1/365532/fe6d5b51e4cb/molcellb00070-0581-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/48e1/365532/e8c16da981fd/molcellb00070-0582-a.jpg

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Regulation by the autophosphorylation site in overexpressed pp60c-src.过表达的pp60c-src中自磷酸化位点的调控
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本文引用的文献

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Cell. 1983 Mar;32(3):891-901. doi: 10.1016/0092-8674(83)90074-0.
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Mutagenesis of Fujinami sarcoma virus: evidence that tyrosine phosphorylation of P130gag-fps modulates its biological activity.藤浪肉瘤病毒的诱变：P130gag-fps的酪氨酸磷酸化调节其生物学活性的证据。
Cell. 1984 Jun;37(2):559-68. doi: 10.1016/0092-8674(84)90386-6.
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Isolation of monoclonal antibodies that recognize the transforming proteins of avian sarcoma viruses.
SRC 信号在胰腺癌中的功能作用：最新研究进展为其提供了新的治疗机会。
Oncogene. 2023 Jun;42(22):1786-1801. doi: 10.1038/s41388-023-02701-x. Epub 2023 Apr 29.
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A phospho-tyrosine-based signaling module using SPOP, CSK, and LYN controls TLR-induced IRF activity.一个基于磷酸化酪氨酸的信号转导模块，利用SPOP、CSK和LYN来控制Toll样受体（TLR）诱导的干扰素调节因子（IRF）活性。
Sci Adv. 2022 Jul 8;8(27):eabq0084. doi: 10.1126/sciadv.abq0084.
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Targeting Kinase Signaling in Pancreatic Cancer Stem Cells.靶向胰腺癌干细胞中的激酶信号通路。
Int J Mol Sci. 2020 Oct 9;21(20):7437. doi: 10.3390/ijms21207437.
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Parathyroid hormone-related protein induces fibronectin up-regulation in rat mesangial cells through reactive oxygen species/Src/EGFR signaling.甲状旁腺激素相关蛋白通过活性氧/Src/EGFR 信号通路诱导大鼠系膜细胞纤连蛋白的上调。
Biosci Rep. 2019 Apr 26;39(4). doi: 10.1042/BSR20182293. Print 2019 Apr 30.
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Vanadium Compounds as Pro-Inflammatory Agents: Effects on Cyclooxygenases.钒化合物作为促炎剂：对环氧化酶的影响
Int J Mol Sci. 2015 Jun 4;16(6):12648-68. doi: 10.3390/ijms160612648.
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In vitro membrane reconstitution of the T-cell receptor proximal signaling network.体外重构 T 细胞受体近端信号网络。
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Local mutagenesis of Rous sarcoma virus: the major sites of tyrosine and serine phosphorylation of pp60src are dispensable for transformation.劳氏肉瘤病毒的局部诱变：pp60src 酪氨酸和丝氨酸磷酸化的主要位点对于转化并非必需。
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Replacement of insulin receptor tyrosine residues 1162 and 1163 compromises insulin-stimulated kinase activity and uptake of 2-deoxyglucose.胰岛素受体酪氨酸残基1162和1163的替换会损害胰岛素刺激的激酶活性以及2-脱氧葡萄糖的摄取。
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