Biofuels Unit, Energy Department-CIEMAT, Avda. Complutense 40, 28040 Madrid, Spain.
Biofuels Unit, Energy Department-CIEMAT, Avda. Complutense 40, 28040 Madrid, Spain.
Bioresour Technol. 2014 Apr;158:262-8. doi: 10.1016/j.biortech.2014.02.041. Epub 2014 Feb 20.
A pretreatment that combines a thermo-mechanical process (extrusion) with chemical and biological catalysts to produce fermentable sugars from barley straw (BS) biomass was investigated. BS was firstly extruded with alkali and then, the pretreated material (extrudate) was submitted to extrusion with hydrolytic enzymes (bioextrusion). The bioextrudate was found to have 35% (w/w dwb) of total solids in soluble form, partly coming from carbohydrate hydrolysis during bioextrusion. About 48% of soluble solids dry weight is comprised by sugars, mostly glucose and xylose. Further enzymatic hydrolysis of bioextrudate could be successfully carried out at high solid loading level of 30% (w/v), with sugar production yield of 32 g glucose and 18 g xylose/100g bioextrudate at 72 h incubation (equivalent to 96 and 52 g/l concentration, respectively). These results, together with the high level of integration of the process, indicate a great potential of this pretreatment technology for sugar production from lignocellulosic substrates.
研究了一种预处理方法，该方法将热机械过程（挤压）与化学和生物催化剂结合使用，从大麦秸秆（BS）生物质中生产可发酵糖。首先用碱挤压 BS，然后将预处理材料（挤压物）用水解酶进行挤压（生物挤压）。发现生物挤压物中 35%（w/w dwb）的总固体以可溶形式存在，部分来自生物挤压过程中的碳水化合物水解。约 48%的可溶性固体干重由糖组成，主要是葡萄糖和木糖。在 30%（w/v）的高固体负荷水平下，进一步进行生物挤压物的酶水解，可以成功进行，在 72 小时孵育时，葡萄糖和木糖的产量分别为 32 g 和 18 g/100g 生物挤压物（分别相当于 96 和 52 g/l 的浓度）。这些结果以及该过程的高度集成表明，该预处理技术在木质纤维素底物的糖生产方面具有很大的潜力。
