Jiangsu Key Laboratory of Biofunctional Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, China.
Ten D Nurse Station, Nanjing BenQ Hospital Co., Ltd., Nanjing 210019, China.
Biosens Bioelectron. 2014 Aug 15;58:9-16. doi: 10.1016/j.bios.2014.02.024. Epub 2014 Feb 22.
Sensitive determination of carcinoembryonic antigen (CEA) is very important in clinical research and diagnosis. Herein we report the design and synthesis of a new kind of immunosensor based on the benefits of hyperbranched structure. The hyperbranched polyester was grafted to the surface of indium tin oxides glass (ITO) electrode, and the grafting processes were characterized by attentuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). After CEA and horse radish peroxidase (HRP)-labeled antibody-conjugated AuNPs (HRP-Ab2-AuNPs) bioconjugates were immobilized on the surface of the hyperbranched structure-modified electrode, the optimized conditions of the above electrode were investigated. Moreover, the analytical performance of the proposed immunosensor showed a high sensitivity, a linear range from 0.01 to 80ng/mL with a low detection limit of 2.36pg/mL, and good selectivity for CEA. The designed immunoassay system holds great potential for ultrasensitive electrochemical biosensing of other analytes.
癌胚抗原（CEA）的灵敏检测在临床研究和诊断中非常重要。本文报道了一种基于超支化结构优势的新型免疫传感器的设计和合成。超支化聚酯被接枝到氧化铟锡（ITO）玻璃电极表面，接枝过程通过衰减全反射傅里叶变换红外光谱（ATR-FTIR）、X 射线光电子能谱（XPS）和原子力显微镜（AFM）进行了表征。在 CEA 和辣根过氧化物酶（HRP）标记的抗体-金纳米粒子（HRP-Ab2-AuNPs）生物缀合物固定在超支化结构修饰电极表面后，对上述电极的优化条件进行了研究。此外，所提出的免疫传感器的分析性能表现出高灵敏度，线性范围为 0.01 至 80ng/mL，检测限低至 2.36pg/mL，对 CEA 具有良好的选择性。该设计的免疫分析系统有望用于其他分析物的超灵敏电化学生物传感。
