Department of Chemistry, Faculty of Science, University Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia.
Department of Chemistry, Faculty of Science, University Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia; Functional Device Laboratory, Institute of Advanced Technology, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
Biosens Bioelectron. 2017 Mar 15;89(Pt 1):673-680. doi: 10.1016/j.bios.2015.12.030. Epub 2015 Dec 15.
In this study, a disposable and simple electrochemical immunosensor was fabricated for the detection of carcinoembryonic antigen. In this method, silver nanoparticles (AgNPs) were mixed with reduced graphene oxide (rGO) to modify the surface of screen-printed carbon electrode (SPE). Initially, AgNPs-rGO modified-SPEs were fabricated by using simple electrochemical deposition method. Then the carcinoembryonic antigen (CEA) was immobilized between the primary antibody and horseradish peroxidase (HRP)-conjugated secondary antibody onto AgNPs-rGO modified-SPEs to fabricate a sandwich-type electrochemical immunosensor. The proposed method could detect the CEA with a linear range of 0.05-0.50µgmL and a detection limit down to 0.035µgmL as compared to its non-sandwich counterpart, which yielded a linear range of 0.05-0.40µgmL, with a detection limit of 0.042µgmL. The immunosensor showed good performance in the detection of carcinoembryonic antigen, exhibiting a simple, rapid and low-cost. The immunosensor showed a higher sensitivity than an enzymeless sensor.
本研究制备了一种用于检测癌胚抗原的一次性、简单的电化学免疫传感器。在该方法中，将银纳米粒子（AgNPs）与还原氧化石墨烯（rGO）混合以修饰丝网印刷碳电极（SPE）的表面。首先，通过简单的电化学沉积方法制备 AgNPs-rGO 修饰-SPE。然后，将癌胚抗原（CEA）固定在初级抗体和辣根过氧化物酶（HRP）标记的次级抗体之间，在 AgNPs-rGO 修饰-SPE 上构建三明治型电化学免疫传感器。与非三明治型相比，该方法可检测到 0.05-0.50μgmL 范围内的 CEA，检测限低至 0.035μgmL，而非三明治型的线性范围为 0.05-0.40μgmL，检测限为 0.042μgmL。该免疫传感器在癌胚抗原检测方面表现出良好的性能，具有简单、快速和低成本的特点。与无酶传感器相比，该免疫传感器具有更高的灵敏度。
