[快速特异性IgE检测（免疫CAP®快速法）及皮肤点刺试验在过敏性疾病诊断中的应用]

[Rapid specific IgE assay (ImmunoCAP® RAPID) and skin prick test in the diagnosis of allergic disease].

作者信息

Komiyama Kenichiro, Masumoto Ai, Nishihara Fuyumi, Kobayashi Takehito, Soma Tomoyuki, Hagiwara Koichi, Kanazawa Minoru, Nagata Makoto

机构信息

Department of Respiratory Medicine, Saitama Medical University; Allergy Center, Saitama Medical University.

Department of Respiratory Medicine, Saitama Medical University.

出版信息

Arerugi. 2013 Dec;62(12):1631-41.

PMID:24608652

Abstract

BACKGROUND

ImmunoCAP® Rapid is a rapid test kit to measure the allergen-specific IgE to the eight major inhalation allergen (cat, mite, orchard grass, ragweed, wormwood, dog, cockroach, Japan cedar).

METHODS

We performed ImmunoCAP® Rapid 83 patients with allergic disease (26 males, 57 females, median aged 43 years, 53 of asthma, 43 of allergic rhinitis) in our allergy center. ImmunoCAP® Rapid results were compared with those of skin prick test (SPT).

RESULTS

Although total positive allergens of SPT were higher than that of ImmunoCAP® Rapid (26.5% vs 22.5%, p<0.05), there was no significantly difference of each positive allergen between two tests. The rate of ImmunoCAP® Rapid to Japan cedar was almost equivalent to SPT in all patients (68.7% vs 55.4%, p=0.07). In contrast, the rate of ImmunoCAP® Rapid to Japan cedar was higher than SPT in patients with rhinitis (90.4% vs 71.4%, p<0.05). Efficiency between ImmunoCAP® Rapid and SPT was 86.4%, sensitivity was 66.9%, and specificity was 93.4%. The reactivity of ImmunoCAP® Rapid to allergens significantly correlated with sizes of SPT (erythema: r=0.645, urticaria: r=0.657).

CONCLUSION

Although identification rate in the screening ImmunoCAP® Rapid slightly inferior to SPT, this test system was useful for diagnosis of Japan cedar and mite.

摘要

背景

ImmunoCAP® Rapid是一种快速检测试剂盒，用于检测对八种主要吸入性过敏原（猫、螨、果园草、豚草、艾草、狗、蟑螂、日本柳杉）的过敏原特异性IgE。

方法

我们在过敏中心对83例过敏性疾病患者（26例男性，57例女性，中位年龄43岁，53例哮喘，43例过敏性鼻炎）进行了ImmunoCAP® Rapid检测。将ImmunoCAP® Rapid的检测结果与皮肤点刺试验（SPT）的结果进行比较。

结果

虽然SPT的总阳性过敏原高于ImmunoCAP® Rapid（26.5%对22.5%，p<0.05），但两种检测方法中各阳性过敏原之间无显著差异。在所有患者中，ImmunoCAP® Rapid对日本柳杉的检测率与SPT几乎相当（68.7%对55.4%，p=0.07）。相比之下，在鼻炎患者中，ImmunoCAP® Rapid对日本柳杉的检测率高于SPT（90.4%对71.4%，p<0.05）。ImmunoCAP® Rapid与SPT之间的效率为86.4%，敏感性为66.9%，特异性为93.4%。ImmunoCAP® Rapid对过敏原的反应性与SPT的大小显著相关（红斑：r=0.645，风团：r=0.657）。