Barnes R M, Johnson P M, Blears J, Harvey M M, Finn R
University Department of Immunology, Royal Liverpool Hospital, UK.
Int Arch Allergy Appl Immunol. 1988;87(2):189-93. doi: 10.1159/000234671.
The antigenic specificity of human serum IgG antibodies reactive with common dietary proteins has been evaluated by competitive binding using a solid-phase immunoassay (ELISA). Antibodies reactive with bovine milk antigens were shown to be reactive predominantly with casein, rather than alpha-lactalbumin, beta-lactoglobulin, gamma-globulin or albumin. Furthermore, sera containing antibodies reactive with bovine casein, wheat gliadin and chicken ovalbumin showed competitive binding only by each respective dietary protein antigen. IgG4 antibodies specifically reactive with ovalbumin, gliadin or casein were also not cross-reactive in competitive binding studies. Furthermore, both IgG2 and IgG4 anti-milk antibodies showed significant inhibition only with bovine casein, and not with alpha-lactalbumin or beta-lactoglobulin. These data are relevant to concepts regarding the immunobiological role of antibodies of the IgG4 isotype.
通过使用固相免疫测定法（酶联免疫吸附测定，ELISA）进行竞争性结合，对与常见膳食蛋白质反应的人血清IgG抗体的抗原特异性进行了评估。结果显示，与牛乳抗原反应的抗体主要与酪蛋白反应，而非与α-乳白蛋白、β-乳球蛋白、γ-球蛋白或白蛋白反应。此外，含有与牛酪蛋白、小麦醇溶蛋白和鸡卵白蛋白反应的抗体的血清，仅在各自相应的膳食蛋白质抗原存在时才表现出竞争性结合。在竞争性结合研究中，与卵白蛋白、醇溶蛋白或酪蛋白特异性反应的IgG4抗体也没有交叉反应。此外，IgG2和IgG4抗乳抗体仅在与牛酪蛋白反应时表现出显著抑制，而与α-乳白蛋白或β-乳球蛋白反应时则无此现象。这些数据与关于IgG4同种型抗体免疫生物学作用的概念相关。
