Barnes R M, Johnson P M, Harvey M M, Blears J, Finn R
University Department of Immunology, Royal Liverpool Hospital, UK.
Int Arch Allergy Appl Immunol. 1988;87(2):184-8. doi: 10.1159/000234670.
The isotype distribution of human IgG antibodies reactive with common dietary proteins has been evaluated in sera from adult patients with the irritable bowel syndrome and with bronchial asthma using a solid-phase immunoassay (ELISA). In both these medical disorders, serum antibodies reactive with ovalbumin or gliadin were restricted predominantly to the IgG4 isotype; however, IgG antibodies reactive with bovine milk antigens, notably casein, were often restricted to both the IgG2 and IgG4 isotypes. A similar serum IgG antibody isotype distribution for these dietary protein antigens was also demonstrated in IgG antibody-positive healthy adults. These data amplify the view that production of antibodies of the IgG4 isotype may reflect a normal immune response to dietary protein antigens presented at mucosal surfaces.
采用固相免疫测定法（酶联免疫吸附测定，ELISA），对肠易激综合征成年患者和支气管哮喘成年患者血清中与常见膳食蛋白发生反应的人IgG抗体的同种型分布进行了评估。在这两种疾病中，与卵清蛋白或麦醇溶蛋白发生反应的血清抗体主要局限于IgG4同种型；然而，与牛乳抗原（尤其是酪蛋白）发生反应的IgG抗体通常局限于IgG2和IgG4同种型。在IgG抗体呈阳性的健康成年人中，也证实了这些膳食蛋白抗原具有相似的血清IgG抗体同种型分布。这些数据进一步证明，IgG4同种型抗体的产生可能反映了对黏膜表面呈现的膳食蛋白抗原的正常免疫反应。
