Cao Ruge, Komura Fusae, Nonaka Airi, Kato Takeshi, Fukumashi Junji, Matsui Toshiro
Division of Bioresources and Biosciences, Faculty of Agriculture, Graduate School of Kyushu University.
Anal Sci. 2014;30(3):383-8. doi: 10.2116/analsci.30.383.
This study works on D-(+)-glucose quantitative analysis using diffusion ordered-quantitative (1)H nuclear magnetic resonance spectroscopy (DOSY-qNMR), by which an analyte could be distinguished from interferences based upon a characteristic diffusion coefficient (D) in gradient magnetic fields. The D value of D-(+)-glucose in deuterium oxide at 30°C was 5.6 × 10(-10) m(2)/s at a field gradient pulse of between 5.0 × 10(-2) and 3.0 × 10(-1) T/m, distinguished from fructose, sucrose and starch. Good linearity (r(2) = 0.9998) was obtained between D-(+)-glucose (0.5-20.0 g/L) and the ratio of the resonance area of α-C1 proton (5.21 ppm) in D-(+)-glucose to that of the β-C1 proton (5.25 ppm) in D-glucuronic acid (50.0 g/L) as an internal standard. The DOSY-qNMR method was successfully applied to quantify D-(+)-glucose in orange juice (18.3 ± 1.0 g/L), apple juice (26.3 ± 0.4 g/L) and grape juice (45.6 ± 0.6 g/L); the values agreed well with a conventional F-kit glucose method.
本研究采用扩散排序定量氢核磁共振波谱法（DOSY-qNMR）对D-(+)-葡萄糖进行定量分析，该方法可根据分析物在梯度磁场中的特征扩散系数（D）将其与干扰物区分开来。在30°C的氧化氘中，当场梯度脉冲在5.0×10(-2)至3.0×10(-1) T/m之间时，D-(+)-葡萄糖的D值为5.6×10(-10) m(2)/s，与果糖、蔗糖和淀粉不同。D-(+)-葡萄糖（0.5-20.0 g/L）与作为内标的D-葡萄糖醛酸（50.0 g/L）中α-C1质子（5.21 ppm）的共振面积与β-C1质子（5.25 ppm）的共振面积之比之间具有良好的线性关系（r(2)=0.9998）。DOSY-qNMR方法成功应用于橙汁（18.3±1.0 g/L）、苹果汁（26.3±0.4 g/L）和葡萄汁（45.6±0.6 g/L）中D-(+)-葡萄糖的定量分析；所得值与传统的F试剂盒葡萄糖法结果吻合良好。
