Dos Santos M, Clairand I, Gruel G, Barquinero J F, Incerti S, Villagrasa C
Institut de Radioprotection et de Sureté Nucléaire, IRSN, BP17, Fontenay aux roses 92962, France.
Centre d'Etudes Nucléaires de Bordeaux Gradignan, CENBG, Université de Bordeaux 1, CNRS/IN2P3, chemin du solarium, BP 120, Gradignan 33175, France.
Radiat Prot Dosimetry. 2014 Oct;161(1-4):469-73. doi: 10.1093/rpd/ncu029. Epub 2014 Mar 9.
The purpose of this work is to evaluate the influence of the chromatin condensation on the number of direct double-strand break (DSB) damages induced by ions. Two geometries of chromosome territories containing either condensed or decondensed chromatin were implemented as biological targets in the Geant4 Monte Carlo simulation code and proton and alpha irradiation was simulated using the Geant4-DNA processes. A DBSCAN algorithm was used in order to detect energy deposition clusters that could give rise to single-strand breaks or DSBs on the DNA molecule. The results of this study show an increase in the number and complexity of DNA DSBs in condensed chromatin when compared with decondensed chromatin.
这项工作的目的是评估染色质凝聚对离子诱导的直接双链断裂（DSB）损伤数量的影响。在Geant4蒙特卡罗模拟代码中，将包含凝聚或解凝聚染色质的两种染色体区域几何结构作为生物靶标，并使用Geant4-DNA过程模拟质子和α粒子辐照。使用DBSCAN算法来检测可能导致DNA分子上单链断裂或双链断裂的能量沉积簇。这项研究的结果表明，与解凝聚染色质相比，凝聚染色质中DNA双链断裂的数量和复杂性有所增加。
