Saffrey M J, Burnstock G
Department of Anatomy and Developmental Biology, University College London, United Kingdom.
Cell Tissue Res. 1988 Oct;254(1):167-76. doi: 10.1007/BF00220030.
The gross morphology and growth patterns of substance P, enkephalin-, somatostatin- and vasoactive intestinal peptide-immunoreactive neurons have been studied in explant cultures of the myenteric plexus taken from beneath the newborn guinea-pig taenia coli, grown for up to 4 weeks in vitro. Substance P- and enkephalin-immunoreactive neurons were more abundant than somatostatin- and vasoactive intestinal peptide-immunoreactive neurons. The peptide-containing neuronal cell bodies were clearly visible in culture and exhibited characteristic gross morphologies similar to those described in situ, although some overlap of shape between populations containing different peptides was seen. All four types of peptide-containing fibres were found in the outgrowth and central areas of the cultures. In the case of substance P and somatostatin, the density and pattern of labelling in the central, neuronal area of the cultures resembled that previously seen in the myenteric plexus of the newborn guinea-pig caecum in situ, while the density of the enkephalin-immunoreactive fibres was greater, and that of the vasoactive intestinal peptide-immunoreactive fibres less than that seen in situ. These observations suggest that subpopulations of myenteric neurons containing different peptides may be differentially affected by the culture environment. Possible contributory factors are discussed.
在取自新生豚鼠结肠带下方的肌间神经丛外植体培养物中，对P物质、脑啡肽、生长抑素和血管活性肠肽免疫反应性神经元的大体形态和生长模式进行了研究，这些培养物在体外生长长达4周。P物质和脑啡肽免疫反应性神经元比生长抑素和血管活性肠肽免疫反应性神经元更为丰富。含肽的神经元细胞体在培养物中清晰可见，呈现出与原位描述相似的特征性大体形态，尽管在含不同肽的群体之间可见一些形状重叠。在培养物的生长和中心区域发现了所有四种含肽纤维。就P物质和生长抑素而言，培养物中心神经元区域的标记密度和模式类似于先前在新生豚鼠盲肠肌间神经丛原位观察到的情况，而脑啡肽免疫反应性纤维的密度更大，血管活性肠肽免疫反应性纤维的密度小于原位观察到的情况。这些观察结果表明，含有不同肽的肌间神经元亚群可能受到培养环境的不同影响。讨论了可能的促成因素。
