Dermatology Branch; Center for Cancer Research; National Cancer Institute; Bethesda, MD USA.
Cancer Biol Ther. 2014 Jun 1;15(6):675-7. doi: 10.4161/cbt.28414. Epub 2014 Mar 11.
Induced pluripotent stem cells (iPSCs) are somatic cells that are reprogrammed into a state resembling embryonic stem cells (ESCs). iPSCs represent a promising technology with applications in cancer research, yet current methods used to generate iPSCs limit their translation to clinical use. In a recent Nature article, Obokata et al. detail a novel technique to generate pluripotent murine cells called stimulus-triggered acquisition of pluripotency (STAP). STAP eliminates the need for exogenous expression of reprogramming factors used in previous iPSC technologies, instead transforming somatic cells to pluripotency using physical and chemical stimuli. The authors found that STAP cells are generated at a 10-fold higher efficiency than prior iPSC technologies. STAP cells display several features of pluripotency, namely the expression of pluripotency-related genes (Oct4, Nanog, Sox2, Ecat1, Esg1, and Dax1), the ability to form teratomas in vivo, and the ability to produce viable, fertile mice in blastocyst complementation assays. Here, we review these findings on STAP and contrast it to previous iPSC technologies, while noting the potential of this method to generate autologous anti-tumor immune cells for cancer therapy.
诱导多能干细胞（iPSCs）是一种被重新编程为类似于胚胎干细胞（ESCs）状态的体细胞。iPSCs 是一种有前途的技术，可应用于癌症研究，但目前用于生成 iPSCs 的方法限制了其向临床应用的转化。在最近的一篇《自然》杂志文章中，Obokata 等人详细介绍了一种称为刺激触发多能性获得（STAP）的新型技术，用于生成多能性的小鼠细胞。STAP 消除了在外源表达以前的 iPSC 技术中使用的重编程因子的需要，而是使用物理和化学刺激将体细胞转化为多能性。作者发现，STAP 细胞的生成效率比以前的 iPSC 技术高 10 倍。STAP 细胞表现出多种多能性特征，即多能性相关基因（Oct4、Nanog、Sox2、Ecat1、Esg1 和 Dax1）的表达、体内形成畸胎瘤的能力以及在胚泡互补测定中产生有活力、可育小鼠的能力。在这里，我们回顾了关于 STAP 的这些发现，并将其与以前的 iPSC 技术进行了对比，同时注意到该方法在生成用于癌症治疗的自体抗肿瘤免疫细胞方面的潜力。
