Gradinaru Sinziana, Popescu Laura Madalina, Piticescu Roxana Mioara, Zurac Sabina, Ciuluvica Radu, Burlacu Alexandrina, Tutuianu Raluca, Valsan Sorina-Nicoleta, Motoc Adrian Mihail, Voinea Liliana Mary
Ophthalmology Department, "Carol Davila" University of Medicine and Pharmacy, 050474 Bucharest, Romania.
National R & D Institute for Non-ferrous and Rare Metals, 077145 Ilfov, Romania.
Nanomaterials (Basel). 2016 Jan 7;6(1):11. doi: 10.3390/nano6010011.
Cellular uptake and cytotoxicity of nanostructured hydroxyapatite (nanoHAp) are dependent on its physical parameters. Therefore, an understanding of both surface chemistry and morphology of nanoHAp is needed in order to be able to anticipate its behavior. The aim of this paper is to characterize an engineered nanoHAp in terms of physico-chemical properties, biocompatibility, and its capability to reconstitute the orbital wall fractures in rabbits. NanoHAp was synthesized using a high pressure hydrothermal method and characterized by physico-chemical, structural, morphological, and optical techniques. X-ray diffraction revealed HAp crystallites of 21 nm, while Scanning Electron Microscopy (SEM) images showed spherical shapes of HAp powder. Mean particle size of HAp measured by DLS technique was 146.3 nm. Biocompatibility was estimated by the effect of HAp powder on the adhesion and proliferation of mesenchymal stem cells (MSC) in culture. The results showed that cell proliferation on powder-coated slides was between 73.4% and 98.3% of control cells (cells grown in normal culture conditions). Computed tomography analysis of the preformed nanoHAp implanted in orbital wall fractures, performed at one and two months postoperative, demonstrated the integration of the implants in the bones. In conclusion, our engineered nanoHAp is stable, biocompatible, and may be safely considered for reconstruction of orbital wall fractures.
纳米结构羟基磷灰石（nanoHAp）的细胞摄取和细胞毒性取决于其物理参数。因此，需要了解nanoHAp的表面化学和形态，以便能够预测其行为。本文的目的是从物理化学性质、生物相容性及其修复兔眶壁骨折的能力方面对一种工程化的nanoHAp进行表征。采用高压水热法合成了nanoHAp，并通过物理化学、结构、形态和光学技术对其进行了表征。X射线衍射显示HAp微晶尺寸为21nm，而扫描电子显微镜（SEM）图像显示HAp粉末呈球形。通过动态光散射（DLS）技术测得的HAp平均粒径为146.3nm。通过HAp粉末对培养的间充质干细胞（MSC）的黏附和增殖的影响来评估生物相容性。结果表明，在涂有粉末的载玻片上细胞增殖为对照细胞（在正常培养条件下生长的细胞）的73.4%至98.3%。术后1个月和2个月对植入眶壁骨折处的预制nanoHAp进行计算机断层扫描分析，结果表明植入物已与骨整合。总之，我们的工程化nanoHAp是稳定的、生物相容的，可安全地用于眶壁骨折的修复。
