Melo Karina M, Grisolia Cesar K, Pieczarka Julio C, de Souza Ludmilla R, Filho José de Souza, Nagamachi Cleusa Y
Laboratório de Citogenética, Departamento de Genética, Instituto de Ciências Biológicas, Universidade Federal do Pará, Campus do Guamá, Av. Perimetral, sn., Guamá, 66075-900 Belém, PA-Brazil.
Mutagenesis. 2014 May;29(3):215-9. doi: 10.1093/mutage/geu005. Epub 2014 Mar 11.
Aneuploidies are numerical genetic alterations that lead to changes in the normal number of chromosomes due to abnormal segregation during cell division. This type of alteration can occur spontaneously or as a result of exposure to mutagenic agents. The presence of these agents in the environment has increased concern about potential damage to human health. Rotenone, derived from plants of the genera Derris and Lonchocarpus, is a product that is used all over the world as a pesticide and piscicide. Before establishing its potential and efficiency for these purposes, it is essential to know more about the possible adverse effects that it may cause. The current work aimed to evaluate the mutagenic potential of rotenone using fish from the species Oreochromis niloticus, as well as to help in understanding its action mechanism. Our results showed the mutagenic potential of rotenone evidenced by increased formation of micronuclei and nuclear buds at low doses of exposure. The use of fluorescence in situ hybridisation technique made it possible to measure the aneugenic potential of the substance, probably due to its impairment of mitotic spindle formation.
非整倍体是一种数值性遗传改变，由于细胞分裂过程中染色体异常分离，导致染色体正常数目发生变化。这种改变可能自发出现，也可能是接触诱变剂的结果。环境中这些物质的存在增加了人们对其可能对人类健康造成损害的担忧。鱼藤酮是从鱼藤属和豆薯属植物中提取的一种产品，在全世界用作杀虫剂和鱼毒剂。在确定其用于这些目的的潜力和效率之前，有必要更多地了解它可能造成的潜在不利影响。当前的研究旨在利用尼罗罗非鱼评估鱼藤酮的致突变潜力，并有助于了解其作用机制。我们的结果显示，在低剂量暴露时，微核和核芽形成增加证明了鱼藤酮的致突变潜力。荧光原位杂交技术的使用使得测量该物质的非整倍体潜力成为可能，这可能是由于它对有丝分裂纺锤体形成的损害。
