家猫中与主要组织相容性复合体（MHC）相关的微卫星标记的开发及其在评估家猫、猎豹和吉尔狮MHC多样性中的应用。
Development of MHC-Linked Microsatellite Markers in the Domestic Cat and Their Use to Evaluate MHC Diversity in Domestic Cats, Cheetahs, and Gir Lions.
作者信息
Morris Katrina M, Kirby Katherine, Beatty Julia A, Barrs Vanessa R, Cattley Sonia, David Victor, O'Brien Stephen J, Menotti-Raymond Marilyn, Belov Katherine
机构信息
From the Faculty of Veterinary Science, University of Sydney, Sydney, NSW 2006, Australia (Morris, Kirby, Beatty, Barrs, and Belov); the ANGIS, University of Sydney, Sydney, NSW 2006, Australia (Cattley); the Laboratory of Genomic Diversity, National Cancer Institute, Frederick, MD 21702-1201 (David and Menotti-Raymond); the Theodosius Dobzhansky Center for Genome Bioinformatics, St. Petersburg State University, St. Petersburg, Russia (O'Brien); and the Oceanographic Center, Nova Southeastern University, Ft Lauderdale, FL 33314-7796 (O'Brien).
出版信息
J Hered. 2014 Jul-Aug;105(4):493-505. doi: 10.1093/jhered/esu017. Epub 2014 Mar 11.
Diversity within the major histocompatibility complex (MHC) reflects the immunological fitness of a population. MHC-linked microsatellite markers provide a simple and an inexpensive method for studying MHC diversity in large-scale studies. We have developed 6 MHC-linked microsatellite markers in the domestic cat and used these, in conjunction with 5 neutral microsatellites, to assess MHC diversity in domestic mixed breed (n = 129) and purebred Burmese (n = 61) cat populations in Australia. The MHC of outbred Australian cats is polymorphic (average allelic richness = 8.52), whereas the Burmese population has significantly lower MHC diversity (average allelic richness = 6.81; P < 0.01). The MHC-linked microsatellites along with MHC cloning and sequencing demonstrated moderate MHC diversity in cheetahs (n = 13) and extremely low diversity in Gir lions (n = 13). Our MHC-linked microsatellite markers have potential future use in diversity and disease studies in other populations and breeds of cats as well as in wild felid species.
主要组织相容性复合体（MHC）内的多样性反映了一个种群的免疫适应性。与MHC连锁的微卫星标记为大规模研究MHC多样性提供了一种简单且廉价的方法。我们在家猫中开发了6个与MHC连锁的微卫星标记，并将这些标记与5个中性微卫星一起用于评估澳大利亚家猫混种（n = 129）和纯种缅甸猫（n = 61）群体中的MHC多样性。澳大利亚杂种猫的MHC具有多态性（平均等位基因丰富度 = 8.52），而缅甸猫群体的MHC多样性显著较低（平均等位基因丰富度 = 6.81；P < 0.01）。与MHC连锁的微卫星以及MHC克隆和测序表明，猎豹（n = 13）的MHC多样性中等，而吉尔狮（n = 13）的MHC多样性极低。我们与MHC连锁的微卫星标记在未来有可能用于其他猫种群和品种以及野生猫科动物的多样性和疾病研究。
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