Rajamani Karthyayani, Li Yuan-Sheng, Hsieh Dean-Kuo, Lin Shinn-Zong, Harn Horng-Jyh, Chiou Tzyy-Wen
Department of Life Science and Graduate Institute of Biotechnology, National Dong Hwa University, Hualien, Taiwan.
Cell Transplant. 2014;23(4-5):417-33. doi: 10.3727/096368914X678472. Epub 2014 Mar 11.
Recently, research on stem cells has been receiving an increasing amount of attention, both for its advantages and disadvantages. Genetic and epigenetic instabilities among stem cells have been a recurring obstacle to progress in regenerative medicine using stem cells. Various reports have stated that these instabilities can transform stem cells when transferred in vivo and thus have the potential to develop tumors. Previous research has shown that various extrinsic and intrinsic factors can contribute to the stability of stem cells. The extrinsic factors include growth supplements, growth factors, oxygen tension, passage technique, and cryopreservation. Controlling these factors based on previous reports may assist researchers in developing strategies for the production and clinical application of "safe" stem cells. On the other hand, the intrinsic factors can be unpredictable and uncontrollable; therefore, to ensure the successful use of stem cells in regenerative medicine, it is imperative to develop and implement appropriate strategies and technique for culturing stem cells and to confirm the genetic and epigenetic safety of these stem cells before employing them in clinical trials.
最近，干细胞研究因其利弊受到了越来越多的关注。干细胞中的基因和表观遗传不稳定性一直是利用干细胞的再生医学进展中反复出现的障碍。各种报告指出，这些不稳定性在体内转移时可使干细胞发生转化，因此有可能引发肿瘤。先前的研究表明，多种外在和内在因素可影响干细胞的稳定性。外在因素包括生长补充剂、生长因子、氧张力、传代技术和冷冻保存。根据先前的报告控制这些因素可能有助于研究人员制定“安全”干细胞的生产和临床应用策略。另一方面，内在因素可能不可预测且无法控制；因此，为确保干细胞在再生医学中的成功应用，必须制定并实施合适的干细胞培养策略和技术，并在将这些干细胞用于临床试验之前确认其基因和表观遗传安全性。
