El-Faham Ayman, Elzatahry Ahmed A, Al-Othman Zeid A, Elsayed Elsayed Ahmed
Department of Chemistry, College of Science, King Saud University, Riyadh, Saudi Arabia ; Department of Chemistry, Faculty of Science, Alexandria University, Ibrahimia, Alexandria, Egypt.
Department of Chemistry, College of Science, King Saud University, Riyadh, Saudi Arabia ; Polymer Materials Research Department, Advanced Technology and New Materials Research Institute, City of Scientific Research and Technology Applications, New Borg El-Arab City, Alexandria, Egypt.
Int J Nanomedicine. 2014 Mar 5;9:1167-74. doi: 10.2147/IJN.S58571. eCollection 2014.
A new method for preparation of silver nanoparticles (AgNPs) based on using hydrazino-isatin derivatives in an aqueous methanol reaction medium is reported here. AgNPs were prepared using silver nitrate solubilized in a water core as the source of silver ions and 3-hydrazino-isatin derivatives (3-hydrazino-isatin [IsH] and 1-benzyl-3-hydrazino-isatin [BIsH]) solubilized in methanol core as a reducing agent. The proposed method is effective, rapid, and convenient. X-ray diffraction (XRD), energy dispersive X-ray analysis, scanning electron microscope (SEM) and transmission electron microscopy (TEM) were used for characterization of the AgNPs. The TEM micrographs confirmed that the nanopowders consist of well-dispersed agglomerates of grains with a narrow size distribution of 18-21 nm and 17-20 nm. The AgNPs, as well as BIsH, showed high antimicrobial and bactericidal activity against the Gram-positive Bacillus subtilis and Gram-negative Micrococcus luteus and Proteus vulgaris, as well as antifungal activities against Saccharomyces cerevisiae. On the other hand, they were not effective against the Gram-negative Escherichia coli.
A simple, effective, rapid, and convenient chemical reduction method for the synthesis of AgNPs in an aqueous methanol reaction medium using hydrazino-isatin derivatives and studying their antibacterial effect.
IsH and BIsH are remarkably powerful reductants for Ag+ ions in an aqueous methanol medium, which could be considered as a simple chemical reduction method for formation of AgNPs. The AgNP formation depends on the solubility of the hydrazino-isatin derivatives. BIsH gave more AgNPs than IsH, as observed from XRD. The formation of AgNPs is attributed to the adsorption of hydrazine derivatives and/or interparticle interaction on the surface of AgNP through electrostatic interactions between the lone pair electrons of the hydrazino group (C=N-NH2) and the positive surface of AgNPs. AgNPs and BIsH showed high antimicrobial and bacterial activity.
In summary, it is shown that IsH and BIsH are remarkably powerful reductants for Ag+ ions in an aqueous methanol medium. BIsH gave more AgNPs than IsH, as observed from XRD due to better solubility of the BIsH than IsH in aqueous-methanol. The formation of AgNPs is attributed to the adsorption of hydrazine derivatives and/or interparticle interaction on the surface of AgNPs through electrostatic interactions between the lone pair electrons of the hydrazino group (C=N-NH2) and the positive surface of AgNPs. The AgNps as well as BIsH ligand showed high antimicrobial and bactericidal activity.
本文报道了一种基于在甲醇水溶液反应介质中使用肼基异吲哚酮衍生物制备银纳米颗粒（AgNPs）的新方法。以溶解在水核中的硝酸银作为银离子源，以溶解在甲醇核中的3-肼基异吲哚酮衍生物（3-肼基异吲哚酮[IsH]和1-苄基-3-肼基异吲哚酮[BIsH]）作为还原剂来制备AgNPs。所提出的方法有效、快速且方便。使用X射线衍射（XRD）、能量色散X射线分析、扫描电子显微镜（SEM）和透射电子显微镜（TEM）对AgNPs进行表征。TEM显微照片证实，纳米粉末由分散良好的颗粒团聚体组成，粒径分布狭窄，分别为18 - 21 nm和17 - 20 nm。AgNPs以及BIsH对革兰氏阳性枯草芽孢杆菌、革兰氏阴性藤黄微球菌和普通变形杆菌显示出高抗菌和杀菌活性，对酿酒酵母也有抗真菌活性。另一方面，它们对革兰氏阴性大肠杆菌无效。
一种在甲醇水溶液反应介质中使用肼基异吲哚酮衍生物合成AgNPs并研究其抗菌效果的简单、有效、快速且方便的化学还原方法。
在甲醇水溶液介质中，IsH和BIsH是Ag⁺离子非常强的还原剂，这可被视为一种形成AgNPs的简单化学还原方法。AgNP的形成取决于肼基异吲哚酮衍生物的溶解度。从XRD观察到，BIsH比IsH生成更多的AgNPs。AgNPs的形成归因于肼衍生物的吸附和/或通过肼基（C=N-NH₂）的孤对电子与AgNPs的正表面之间的静电相互作用在AgNP表面的颗粒间相互作用。AgNPs和BIsH显示出高抗菌和杀菌活性。
总之，结果表明在甲醇水溶液介质中，IsH和BIsH是Ag⁺离子非常强的还原剂。如从XRD观察到的，由于BIsH在甲醇 - 水体系中的溶解度比IsH好，BIsH比IsH生成更多的AgNPs。AgNPs的形成归因于肼衍生物的吸附和/或通过肼基（C=N-NH₂） 的孤对电子与AgNPs的正表面之间的静电相互作用在AgNP表面的颗粒间相互作用。AgNps以及BIsH配体显示出高抗菌和杀菌活性。
