Jung Hyun Ah, Kim Jae-I, Choung Se Young, Choi Jae Sue
Department of Food Science and Human Nutrition, Chonbuk National University, Jeonju, Korea.
J Pharm Pharmacol. 2014 Aug;66(8):1180-8. doi: 10.1111/jphp.12241. Epub 2014 Mar 13.
As part of our efforts to isolate anti-hepatotoxic agents from marine natural products, we screened the ability of 14 edible varieties of Korean seaweed to protect against doxorubicin-induced hepatotoxicity in primary rat hepatocytes.
Among the crude extracts of two Chlorophyta (Codium fragile and Capsosiphon fulvescens), seven Phaeophyta (Undaria pinnatifida, Sargassum thunbergii, Pelvetia siliquosa, Ishige okamurae, Ecklonia cava, Ecklonia stolonifera and Eisenia bicyclis), five Rhodophyta (Chondrus ocellatus, Gelidium amansii, Gracilaria verrucosa, Symphycladia latiuscula and Porphyra tenera), and the extracts of Ecklonia stolonifera, Ecklonia cava, Eisenia bicyclis and Pelvetia siliquosa exhibited significant protective effects on doxorubicin-induced hepatotoxicity, with half maximal effective concentration (EC50) values of 2.0, 2.5, 3.0 and 15.0 μg/ml, respectively.
Since Ecklonia stolonifera exhibits a significant protective potential and is frequently used as foodstuff, we isolated six phlorotannins, including phloroglucinol (1), dioxinodehydroeckol (2), eckol (3), phlorofucofuroeckol A (4), dieckol (5) and triphloroethol-A (6). Phlorotannins 2 ∼ 6 exhibited potential protective effects on doxorubicin-induced hepatotoxicity, with corresponding EC50 values of 3.4, 8.3, 4.4, 5.5 and 11.5 μg/ml, respectively.
The results clearly demonstrated that the anti-hepatotoxic effects of Ecklonia stolonifera and its isolated phlorotannins are useful for further exploration and development of therapeutic modalities for treatment of hepatotoxicity.
作为我们从海洋天然产物中分离抗肝毒性剂工作的一部分，我们筛选了14种可食用的韩国海藻对原代大鼠肝细胞中阿霉素诱导的肝毒性的保护能力。
在两种绿藻（柔弱刚毛藻和满二叉藻）、七种褐藻（裙带菜、鼠尾藻、岩藻、冈村石莼、海蕴、匍匐马尾藻和 bicyclis Eisenia）、五种红藻（角叉菜、石花菜、江蓠、宽管藻和紫菜）的粗提物中，匍匐马尾藻、海蕴、bicyclis Eisenia和岩藻的提取物对阿霉素诱导的肝毒性表现出显著的保护作用，半数最大有效浓度（EC50）值分别为2.0、2.5、3.0和15.0μg/ml。
由于匍匐马尾藻具有显著的保护潜力且经常被用作食品，我们分离出了六种间苯三酚单宁，包括间苯三酚（1）、二氧代去氢eckol（2）、eckol（3）、间苯三酚岩藻呋喃醚A（4）、二eckol（5）和三聚间苯三酚-A（6）。间苯三酚单宁2至6对阿霉素诱导的肝毒性表现出潜在的保护作用，相应的EC50值分别为3.4、8.3、4.4、5.5和11.5μg/ml。
结果清楚地表明，匍匐马尾藻及其分离出的间苯三酚单宁的抗肝毒性作用对于进一步探索和开发治疗肝毒性的治疗方法是有用的。
