Kang Hye Sook, Chung Hae Young, Kim Ji Young, Son Byeng Wha, Jung Hyun Ah, Choi Jae Sue
Faculty of Food Science and Biotechnology, Pukyong National University, Busan 608-737, Korea.
Arch Pharm Res. 2004 Feb;27(2):194-8. doi: 10.1007/BF02980106.
Reactive oxygen species (ROS) play an important role in the pathogenesis of many human degenerative diseases such as cancer, aging, arteriosclerosis, and rheumatism. Much attention has been focused on the development of safe and effective antioxidants. To discover sources of antioxidative activity in marine algae, extracts from 17 kinds of seaweed were screened for their inhibitory effect on total ROS generation in kidney homogenate using 2',7'-dichlorofluorescein diacetate (DCFH-DA). ROS inhibition was seen in three species: Ulva pertusa, Symphyocladia latiuscula, and Ecklonia stolonifera. At a final concentration of 25 microg/mL, U. pertusa inhibited 85.65+/-20.28% of total ROS generation, S. latiscula caused 50.63+/-0.09% inhibitory, and the Ecklonia species was 44.30+/-7.33% inhibition. E. stolonifera Okamura (Laminariaceae), which belongs to the brown algae, has been further investigated because it is commonly used as a foodstuff in Korea. Five compounds, phloroglucinol (1), eckstolonol (2), eckol (3), phlorofucofuroeckol A (4), and dieckol (5), isolated from the ethyl acetate soluble fraction of the methanolic extract of E. stolonifera inhibited total ROS generation.
活性氧（ROS）在许多人类退行性疾病如癌症、衰老、动脉硬化和风湿病的发病机制中起重要作用。人们一直非常关注安全有效的抗氧化剂的开发。为了发现海藻中的抗氧化活性来源，使用2'，7'-二氯荧光素二乙酸酯（DCFH-DA）对17种海藻的提取物进行了筛选，以检测它们对肾脏匀浆中总ROS生成的抑制作用。在三种海藻中观察到了ROS抑制作用：孔石莼、宽叶香藻和匍匐马尾藻。在终浓度为25μg/mL时，孔石莼抑制了85.65±20.28%的总ROS生成，宽叶香藻产生了50.63±0.09%的抑制率，而马尾藻属物种的抑制率为44.30±7.33%。属于褐藻的冈村匍匐马尾藻（海带科）因其在韩国常被用作食品而受到进一步研究。从匍匐马尾藻甲醇提取物的乙酸乙酯可溶部分中分离出的五种化合物，间苯三酚（1）、eckstolonol（2）、eckol（3）、phlorofucofuroeckol A（4）和dieckol（5），抑制了总ROS的生成。
