Venter Marietjie, Zaayman Dewald, van Niekerk Stephanie, Stivaktas Voula, Goolab Shivani, Weyer Jacqueline, Paweska Janusz T, Swanepoel Robert
Zoonoses Research Unit, University of Pretoria, Pretoria, South Africa; Centre for Respiratory Diseases and Meningitis, National Institute for Communicable Diseases of the National Health Laboratory Service, Sandringham, South Africa.
Zoonoses Research Unit, University of Pretoria, Pretoria, South Africa.
J Clin Virol. 2014 May;60(1):50-6. doi: 10.1016/j.jcv.2014.02.001. Epub 2014 Feb 14.
Many cases of acute febrile illness with nervous manifestations go undiagnosed, partly because the potential pathogens are not investigated routinely.
To develop a multiplex PCR-based macroarray for detection of 29 pathogens associated with febrile disease, aseptic meningitis and meningoencephalitis in southern Africa, including common viruses, bacteria, parasites and selected arboviruses.
Pathogens were identified by hybridization of PCR amplicons to probes on a macroarray chip, followed by colorimetric detection.
Positive control specimens for all 29 targets were detected with high sensitivity. Twenty-seven clinical samples previously found positive for various etiologies of febrile disease and meningoencephalitis, including less common infections such as Crimean Congo haemorrhagic fever, Rift Valley fever, West Nile and rabies were all identified. Testing of a blinded panel of 16 specimens in triplicate demonstrated high repeatability. Screening of 138 specimens from patients with febrile and/or neurological signs that could not be solved in routine investigations yielded 5 additional diagnoses.
A highly sensitive, specific and easily interpreted test was developed to detect 29 pathogens associated with febrile and nervous disease.
许多伴有神经症状的急性发热性疾病病例未得到诊断，部分原因是未常规检测潜在病原体。
开发一种基于多重PCR的宏阵列，用于检测与非洲南部发热性疾病、无菌性脑膜炎和脑膜脑炎相关的29种病原体，包括常见病毒、细菌、寄生虫和特定虫媒病毒。
通过将PCR扩增产物与宏阵列芯片上的探针杂交，随后进行比色检测来鉴定病原体。
所有29个靶点的阳性对照标本均以高灵敏度检测到。先前发现的27份临床样本对发热性疾病和脑膜脑炎的各种病因呈阳性，包括克里米亚-刚果出血热、裂谷热、西尼罗河病毒和狂犬病等不太常见的感染，均被鉴定出来。对16份标本进行三次重复的盲法检测显示出高重复性。对138例有发热和/或神经症状且在常规检查中无法确诊的患者标本进行筛查，又得出了5个额外的诊断结果。
开发了一种高灵敏度、特异性且易于解读的检测方法，用于检测与发热和神经疾病相关的29种病原体。
