Sainsbury Frank, Lomonossoff George P
The University of Queensland, Australian Institute for Bioengineering and Nanotechnology, Centre for Biomolecular Engineering, St Lucia, QLD 4072, Australia.
Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, United Kingdom.
Curr Opin Plant Biol. 2014 Jun;19(100):1-7. doi: 10.1016/j.pbi.2014.02.003. Epub 2014 Mar 12.
Recent developments in transient expression methods have enabled the efficient delivery and expression of multiple genes within the same plant cell over a timescale of days. In some cases, the vectors deployed can be fine-tuned to allow differential expression of the various genes. This has opened the way to the deployment of transient expression for such applications as the production of macromolecular complexes and the analysis and manipulation of metabolic pathways. The ability to observe the effect of gene expression in a matter of days means that transient expression is becoming the method of choice for many plant-based synthetic biology applications.
瞬时表达方法的最新进展使得在数天的时间尺度内能够在同一植物细胞中高效传递和表达多个基因。在某些情况下，所使用的载体可以进行微调，以实现各种基因的差异表达。这为瞬时表达在诸如大分子复合物生产以及代谢途径分析和操纵等应用中的部署开辟了道路。能够在数天内观察到基因表达的效果意味着瞬时表达正成为许多基于植物的合成生物学应用的首选方法。
