Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK.
New Phytol. 2013 Oct;200(1):16-26. doi: 10.1111/nph.12204. Epub 2013 Mar 4.
This review discusses the varying roles that have been played by many plant-viral regulatory sequences and proteins in the creation of plant-based expression systems and virus particles for use in nanotechnology. Essentially, there are two ways of expressing an exogenous protein: the creation of transgenic plants possessing a stably integrated gene construction, or the transient expression of the desired gene following the infiltration of the gene construct. Both depend on disarmed strains of Agrobacterium tumefaciens to deliver the created gene construction into cell nuclei, usually through the deployment of virus-derived components. The importance of efficient mRNA translation in the latter process is highlighted. Plant viruses replicate to sustain an infection to promote their survival. The major product of this, the virus particle, is finding increasing roles in the emerging field of bionanotechnology. One of the major products of plant-viral expression is the virus-like particle (VLP). These are increasingly playing a role in vaccine development. Similarly, many VLPs are suitable for the investigation of the many facets of the emerging field of synthetic biology, which encompasses the design and construction of new biological functions and systems not found in nature. Genetic and chemical modifications to plant-generated VLPs serve as ideal starter templates for many downstream synthetic biology applications.
本文讨论了许多植物病毒调控序列和蛋白在植物表达系统和病毒颗粒的创建中的不同作用，这些系统和颗粒可用于纳米技术。本质上，有两种表达外源蛋白的方法：创建稳定整合基因构建体的转基因植物，或在基因构建体渗透后瞬时表达所需基因。这两种方法都依赖于无毒性的根瘤农杆菌菌株将创建的基因构建体递送到细胞核中，通常是通过利用病毒衍生的成分。在后一种过程中，高效的 mRNA 翻译的重要性被强调了。植物病毒为了维持感染而进行复制，以促进其生存。该过程的主要产物是病毒颗粒，它在新兴的生物纳米技术领域中发挥着越来越重要的作用。植物病毒表达的主要产物之一是病毒样颗粒（VLP）。这些颗粒在疫苗开发中越来越发挥作用。同样，许多 VLP 适用于新兴的合成生物学领域的许多方面的研究，包括设计和构建自然界中不存在的新的生物功能和系统。对植物产生的 VLP 进行遗传和化学修饰，可作为许多下游合成生物学应用的理想起始模板。
