Gopi D, Shinyjoy E, Kavitha L
Department of Chemistry, Periyar University, Salem 636011, Tamil Nadu, India; Centre for Nanoscience and Nanotechnology, Periyar University, Salem 636011, Tamil Nadu, India.
Department of Chemistry, Periyar University, Salem 636011, Tamil Nadu, India; Centre for Nanoscience and Nanotechnology, Periyar University, Salem 636011, Tamil Nadu, India.
Spectrochim Acta A Mol Biomol Spectrosc. 2014 Jun 5;127:286-91. doi: 10.1016/j.saa.2014.02.057. Epub 2014 Feb 26.
The present work is aimed at the synthesis of antibacterial and bioactive silver/magnesium co-substituted hydroxyapatite (Ag/Mg-HAP) powders. For this purpose, firstly, different concentrations (0.5, 1.5, 2.5wt.%) of silver substituted HAP (Ag-HAP) powders were prepared by ultrasonic irradiation technique and were characterized by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM) and energy dispersive X-ray analysis (EDAX). Secondly, magnesium (Mg) is co-substituted as secondary material into Ag-HAP to offset the potential cytotoxicity of Ag, as higher concentration of Ag is toxic. The antibacterial activity of as-synthesized powders was evaluated by Escherichia coli (E. coli) and was found to be effectively high against bacterial colonization. Also, the in vitro cell-material interaction is evaluated with human osteosarcoma MG63 (HOS MG63) cells for cell proliferation. The results showed the evidence of cytotoxic effects of the higher concentration of Ag-HAP characterized by poor cellular viability whereas, Ag/Mg-HAP showed better cell viability indicating that co-substitution of Mg in Ag-HAP effectively offset the negative effects of Ag and improve performance compared with pure HAP. Thus, the as synthesized Ag/Mg-HAP will serve as a better candidate for biomedical applications with good antibacterial property and bone bonding ability.
本研究旨在合成具有抗菌和生物活性的银/镁共取代羟基磷灰石（Ag/Mg-HAP）粉末。为此，首先，通过超声辐照技术制备了不同浓度（0.5、1.5、2.5wt.%）的银取代羟基磷灰石（Ag-HAP）粉末，并通过傅里叶变换红外光谱（FTIR）、X射线衍射（XRD）、扫描电子显微镜（SEM）和能量色散X射线分析（EDAX）对其进行了表征。其次，将镁（Mg）作为二次材料共取代到Ag-HAP中，以抵消银的潜在细胞毒性，因为高浓度的银具有毒性。通过大肠杆菌（E. coli）评估了合成粉末的抗菌活性，发现其对细菌定植具有高效的抗菌作用。此外，还用人骨肉瘤MG63（HOS MG63）细胞评估了体外细胞与材料的相互作用，以检测细胞增殖情况。结果表明，高浓度Ag-HAP具有细胞毒性作用，表现为细胞活力较差，而Ag/Mg-HAP显示出较好的细胞活力，这表明在Ag-HAP中共取代Mg有效地抵消了Ag的负面影响，与纯HAP相比，性能得到了改善。因此，合成的Ag/Mg-HAP将作为一种具有良好抗菌性能和骨结合能力的生物医学应用的更佳候选材料。
