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采用电泳沉积法在 Ti-6Al-7Nb 合金上制备铜纳米颗粒涂层的成骨细胞反应。

Osteoblastic Cell Responses of Copper Nanoparticle Coatings on Ti-6Al-7Nb Alloy Using Electrophoretic Deposition Method.

机构信息

Prosthdontic Department, College of Dentistry, University of Babylon, Iraq.

Oral and Maxillofacial Surgery Department, College of Dentistry, University of Babylon, Iraq.

出版信息

Biomed Res Int. 2022 Apr 19;2022:3675703. doi: 10.1155/2022/3675703. eCollection 2022.

DOI:10.1155/2022/3675703
PMID:35496039
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9042614/
Abstract

AIM

To investigate and compare the cell cytotoxicity, proliferation, cell attachment, and morphology of human fetal osteoblasts (hFOB) cells of coated samples (titanium nanocopper (Ti Cu), titanium nanohydroxyapatite (Ti HA) and titanium nanocopper ion doped hydroxyapatite (Ti Cu/HA) and uncoated samples (Ti) in order to assess the suitability of these surface modifications on Ti-6Al-7Nb for dental implant application.

MATERIALS AND METHODS

The cytotoxicity was studied by examining the hFOB cell response by MTT assessment. The cell morphology was evaluated by inverted microscopy and observed under scanning electronic microscopy (SEM).

RESULTS

MTT assay results displayed that the Cu content on the surface of Ti-6Al-7Nb alloys did not produce any cytotoxic effect on cell viability. The cell viability rate in all samples ranges from 97% to 126%, indicating that hFOB cells grew at a high proliferation rate. However, no significant differences in cell viability were observed between Ti and Ti Cu and between Ti HA and Ti Cu/HA groups. Microscopic examination demonstrated no difference in the cell morphology of hFOB among all samples. In addition, SEM observation indicated favorable adhesion and spreading of the cells on the coated and uncoated samples.

CONCLUSIONS

The surface modification of Ti-6Al-7Nb alloy with Cu, HA, and Cu/HA exhibits good cell biocompatibility, and the Cu has no influence on the cell proliferation and differentiation of hFOB.

摘要

目的

研究并比较涂层样品（钛纳米铜（TiCu）、钛纳米羟基磷灰石（TiHA）和钛纳米铜离子掺杂羟基磷灰石（TiCu/HA）和未涂层样品（Ti）对人胎成骨细胞（hFOB）细胞的细胞毒性、增殖、细胞黏附及形态的影响，以评估这些表面改性对钛-6 铝-7 铌用于牙科植入物的适用性。

材料和方法

通过 MTT 评估检查 hFOB 细胞反应来研究细胞毒性。通过倒置显微镜观察和扫描电子显微镜（SEM）观察评估细胞形态。

结果

MTT 检测结果显示，Ti-6Al-7Nb 合金表面的 Cu 含量对细胞活力没有产生任何细胞毒性作用。所有样品中的细胞活力率范围在 97%到 126%之间，表明 hFOB 细胞具有较高的增殖率。然而，Ti 和 TiCu 之间以及 TiHA 和 TiCu/HA 之间的细胞活力没有显著差异。显微镜检查显示，hFOB 的细胞形态在所有样品中没有差异。此外，SEM 观察表明细胞在涂层和未涂层样品上具有良好的黏附和铺展。

结论

Ti-6Al-7Nb 合金表面用 Cu、HA 和 Cu/HA 进行改性表现出良好的细胞生物相容性，Cu 对 hFOB 的细胞增殖和分化没有影响。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1353/9042614/568845cd6714/BMRI2022-3675703.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1353/9042614/bc0650cfe59b/BMRI2022-3675703.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1353/9042614/7b333aa605da/BMRI2022-3675703.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1353/9042614/0ff82de619ad/BMRI2022-3675703.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1353/9042614/42e16e02fd16/BMRI2022-3675703.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1353/9042614/fc01995de64d/BMRI2022-3675703.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1353/9042614/568845cd6714/BMRI2022-3675703.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1353/9042614/bc0650cfe59b/BMRI2022-3675703.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1353/9042614/7b333aa605da/BMRI2022-3675703.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1353/9042614/0ff82de619ad/BMRI2022-3675703.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1353/9042614/42e16e02fd16/BMRI2022-3675703.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1353/9042614/fc01995de64d/BMRI2022-3675703.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1353/9042614/568845cd6714/BMRI2022-3675703.006.jpg

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