Cha In-Tae, Yim Kyung June, Song Hye Seon, Lee Hae-Won, Hyun Dong-Wook, Kim Kil-Nam, Choi Jong-Soon, Kim Daekyung, Lee Sung-Jae, Seo Myung-Ji, Choi Hak-Jong, Bae Jin-Woo, Rhee Sung-Keun, Roh Seong Woon, Nam Young-Do
Jeju Center, Korea Basic Science Institute, Jeju, 690-140, Republic of Korea.
Antonie Van Leeuwenhoek. 2014 May;105(5):907-14. doi: 10.1007/s10482-014-0145-0. Epub 2014 Mar 15.
Two Gram-stain negative, rod-shaped and motile extreme halophiles, designated CBA1107(T) and CBA1108, were isolated from non-purified solar salt. Based on the phylogenetic analysis, strains CBA1107(T) and CBA1108 were shown to belong to the genus Halolamina, with similarities for the 16S rRNA gene sequences between strains CBA1107(T) and Halolamina pelagica TBN21(T) , Halolamina salina WSY15-H3(T) and Halolamina salifodinae WSY15-H1(T) of 98.3, 97.6 and 97.3 %, respectively; the similarities for the rpoB' gene sequences between the same strains were 96.0, 95.3 and 94.6 %, respectively. The colonies of both strains were observed to be red pigmented on growth medium. Strain CBA1107(T) was observed to grow at 20-50 °C, in the presence of 15-30 % NaCl, at pH 6.0-9.0, and with 0.005-0.5 M Mg(2+). The cells of both strains lysed in distilled water. The DNA-DNA hybridization experiments showed that strain CBA1107(T) shared 97 % relatedness with CBA1108 and <50 % relatedness with H. pelagica JCM 16809(T), H. salina JCM 18549(T) and H. salifodinae JCM 18548(T). The genomic DNA G+C content of strain CBA1107(T) was determined to be 65.1 mol%. The major polar lipids of the two strains were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and glycolipids including sulfated mannosyl glucosyl diether and mannosyl glucosyl diether. Based on the polyphasic taxonomic analyses, the strains are considered to represent a new taxon for which the name Halolamina rubra sp. nov. is proposed, with the type strain CBA1107(T) (=CECT 8421(T) =JCM 19436(T)).
从非纯化的日晒盐中分离出两株革兰氏染色阴性、杆状且具运动性的极端嗜盐菌，分别命名为CBA1107(T)和CBA1108。基于系统发育分析，菌株CBA1107(T)和CBA1108被证明属于嗜盐层菌属，菌株CBA1107(T)与浮游嗜盐层菌TBN21(T)、盐生嗜盐层菌WSY15 - H3(T)和盐沼嗜盐层菌WSY15 - H1(T)的16S rRNA基因序列相似性分别为98.3%、97.6%和97.3%；相同菌株间rpoB'基因序列的相似性分别为96.0%、95.3%和94.6%。观察到两株菌在生长培养基上的菌落均呈红色。观察到菌株CBA1107(T)在20 - 50°C、15 - 30% NaCl存在、pH 6.0 - 9.0以及0.005 - 0.5 M Mg(2+)条件下生长。两株菌的细胞在蒸馏水中会裂解。DNA - DNA杂交实验表明，菌株CBA1107(T)与CBA1108的相关性为97%，与浮游嗜盐层菌JCM 16809(T)、盐生嗜盐层菌JCM 18549(T)和盐沼嗜盐层菌JCM 18548(T)的相关性小于50%。菌株CBA1107(T)的基因组DNA G + C含量测定为65.1 mol%。两株菌的主要极性脂为磷脂酰甘油、磷脂酰甘油磷酸甲酯、磷脂酰甘油硫酸酯和糖脂，包括硫酸化甘露糖基葡糖二醚和甘露糖基葡糖二醚。基于多相分类分析，这些菌株被认为代表一个新的分类单元，为此提出新名称红色嗜盐层菌（Halolamina rubra sp. nov.），模式菌株为CBA1107(T)（=CECT 8421(T) =JCM 19436(T)）。
