Koh Hyeon-Woo, Song Hye Seon, Song Uhram, Yim Kyung June, Roh Seong Woon, Park Soo-Je
Department of Biology, Jeju National University, Jejudaehak-ro 102, Jeju 690-756, Republic of Korea.
Biological Disaster Analysis Group, Korea Basic Science Institute, Daejeon 305-806, Republic of Korea.
Int J Syst Evol Microbiol. 2015 Aug;65(8):2479-2484. doi: 10.1099/ijs.0.000287. Epub 2015 Apr 28.
An extremely halophilic archaeal strain, halo-7T, was isolated from brine sediment of the Gomso solar saltern, Republic of Korea. Cells of strain halo-7T were pleomorphic, stained Gram-negative, lysed in distilled water and formed red-pigmented colonies. Strain halo-7T grew in the range of 25-45°C (optimum 37-40°C), pH 6.5-9.5 (optimum pH 7.0-8.0), 15-30% (w/v) NaCl (optimum, 20-25%), and 0.05-0.5 M MgCl2 (optimum 0.1-0.3 M). The minimal NaCl concentration to prevent cell lysis of strain halo-7T was 10% (w/v). The major polar lipids of the isolate were phosphatidylglycerol, phosphatidylglycerolphosphate methyl ester, an unidentified phospholipid, an unidentified lipid, and two unidentified glycolipids. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain halo-7T is closely related to the members of the genus Halolamina, Halolamina salina WSY15-H3T (98.7% 16S rRNA gene sequence similarity), Halolamina pelagica TBN21T (98.2%) and Halolamina rubra CBA1107T (97.4%). The genomic DNA G+C content determined for strain halo-7T (68.0 mol%) was slightly higher than those of H. salina JCM 18549T and H. rubra CBA1107T. DNA-DNA hybridization values between strain halo-7T and reference strains were < 25%. Based on phenotypic, chemotaxonomic and phylogenetic properties, we describe a novel species of the genus Halolamina, represented by strain halo-7T, for which we propose the name Halolamina sediminis sp. nov. The type strain is halo-7T ( = JCM 30187T = CECT 8739T).
从韩国戈姆索太阳能盐场的卤水沉积物中分离出一株极端嗜盐古菌，命名为halo-7T。halo-7T菌株的细胞呈多形性，革兰氏染色阴性，在蒸馏水中会裂解，并形成红色色素菌落。halo-7T菌株在25-45°C（最适温度37-40°C）、pH值6.5-9.5（最适pH值7.0-8.0）、15-30%（w/v）NaCl（最适浓度20-25%）以及0.05-0.5 M MgCl2（最适浓度0.1-0.3 M）的条件下生长。防止halo-7T菌株细胞裂解的最低NaCl浓度为10%（w/v）。该分离株的主要极性脂类为磷脂酰甘油、磷脂酰甘油磷酸甲酯、一种未鉴定的磷脂、一种未鉴定的脂类以及两种未鉴定的糖脂。基于16S rRNA基因序列的系统发育分析表明，halo-7T菌株与盐层嗜盐菌属的成员密切相关，如盐层嗜盐菌WSY15-H3T（16S rRNA基因序列相似性为98.7%）、海洋盐层嗜盐菌TBN21T（98.2%）和红色盐层嗜盐菌CBA1107T（97.4%）。测定的halo-7T菌株基因组DNA的G+C含量（68.0 mol%）略高于盐层嗜盐菌JCM 18549T和红色盐层嗜盐菌CBA1107T。halo-7T菌株与参考菌株之间的DNA-DNA杂交值<25%。基于表型、化学分类和系统发育特性，我们描述了盐层嗜盐菌属的一个新物种，以halo-7T菌株为代表，我们将其命名为盐层沉积物嗜盐菌（Halolamina sediminis sp. nov.）。模式菌株为halo-7T（=JCM 30187T=CECT 8739T）。
