Hao Xiaofeng, Ling Qufei, Hong Fashui
School of Preclinical Medicine and Life Science, Soochow University, 199, Renai Road, Suzhou, 215123, Jiangsu, People's Republic of China.
Fish Physiol Biochem. 2014 Oct;40(5):1313-23. doi: 10.1007/s10695-014-9926-7. Epub 2014 Mar 16.
In this study, loach (Paramisgurnus dabryanus) were fed artificial diets containing 0.31 (control), 0.39, 0.48, 0.50 and 0.62 mg kg(-1) of selenium (Se) for 60 days, respectively. Liver histopathology, hepatocyte ultrastructure, blood indices, biochemical parameters of liver functions and oxidative stress in the Se-treated loach were then assayed. The results showed the following: histopathological and ultrastructural lesions in liver were only observed in loach fed the 0.62 mg Se kg(-1) diet; Haemoglobin and total protein were significantly increased in the 0.50 mg Se kg(-1) group; albumin and high-density lipoprotein were increased significantly in the 0.48-0.50 mg Se kg(-1) groups. However, white blood cell count was significantly decreased in the 0.48 mg Se kg(-1) group; alanine aminotransferase, aspartate aminotransferase and lactate dehydrogenase were decreased in the 0.39-0.50 mg Se kg(-1) groups. In liver tissue, the content of hydrogen peroxide was lower than that of controls in the 0.48-0.50 mg Se kg(-1) groups, and the malondialdehyde level was lowest in the 0.48 mg Se kg(-1) group. The activities of superoxide dismutase and glutathione peroxidase were significantly increased in the 0.50 mg Se kg(-1) group; catalase and total antioxidant capacity were markedly increased in the 0.48-0.50 mg Se kg(-1) group. These present results indicated that the dietary Se requirement for loach is 0.48-0.50 mg Se kg(-1) diet.
在本研究中，将泥鳅（Paramisgurnus dabryanus）分别投喂含0.31（对照）、0.39、0.48、0.50和0.62毫克/千克硒（Se）的人工饲料60天。然后检测了经硒处理的泥鳅的肝脏组织病理学、肝细胞超微结构、血液指标、肝功能生化参数和氧化应激情况。结果如下：仅在投喂0.62毫克硒/千克饲料的泥鳅中观察到肝脏的组织病理学和超微结构损伤；血红蛋白和总蛋白在0.50毫克硒/千克组显著增加；白蛋白和高密度脂蛋白在0.48 - 0.50毫克硒/千克组显著增加。然而，白细胞计数在0.48毫克硒/千克组显著降低；丙氨酸氨基转移酶、天冬氨酸氨基转移酶和乳酸脱氢酶在0.39 - 0.50毫克硒/千克组降低。在肝脏组织中，0.48 - 0.50毫克硒/千克组的过氧化氢含量低于对照组，丙二醛水平在0.48毫克硒/千克组最低。超氧化物歧化酶和谷胱甘肽过氧化物酶的活性在0.50毫克硒/千克组显著增加；过氧化氢酶和总抗氧化能力在0.48 - 0.50毫克硒/千克组显著增加。这些结果表明，泥鳅的饲料硒需求量为0.48 - 0.50毫克硒/千克饲料。
