National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, Sichuan, China.
National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, Sichuan, China.
Biomaterials. 2014 Jun;35(17):4759-68. doi: 10.1016/j.biomaterials.2014.02.041. Epub 2014 Mar 15.
Cell adhesion and spreading are two essential factors for anchorage-dependent cells such as osteocytes. An adhesive macroporous hydrogel system, in which cell-affinitive domains and sufficient cytoskeleton reorganization space were simultaneously constructed, was proposed in this report to support cell adhesion and spreading, respectively, and facilitate cell differentiation and function establishment eventually. The adhesive macroporous alginate hydrogel was developed by RGD peptide graft and gelatin microspheres hybridization to generate cellular adhesion sites and highly interconnected macropores. The successful stretched morphology and enhanced osteogenic differentiation of MG-63 cells in this modified alginate hydrogel showed clearly the feasibility that cell function may be effectively facilitated. Besides, this hydrogel model can be further applied to construct complex micropatterned structure, such as individual microgels in shapes of circle, square, cross and ring, and osteon-like structure containing both osteogenic and vascularized area generated by a double-ring assembly. These results should provide this adhesive macroporous photocrosslinkable hydrogel system as potential three-dimensional scaffolds for guiding tissue formation, especially for the bioengineering of tissues that have multiple cell types and require precisely defined cell-cell and cell-substrate interactions.
细胞黏附和铺展是锚定依赖性细胞（如成骨细胞）的两个重要因素。本报告提出了一种黏附性大孔水凝胶系统，该系统分别构建了细胞亲和结构域和足够的细胞骨架重排空间，以分别支持细胞黏附和铺展，并最终促进细胞分化和功能建立。通过 RGD 肽接枝和明胶微球杂交制备了黏附性大孔海藻酸钠水凝胶，以生成细胞黏附位点和高度互联的大孔。在这种改良的海藻酸钠水凝胶中，MG-63 细胞呈现出明显的拉伸形态和增强的成骨分化，这清楚地表明细胞功能可能得到有效促进。此外，该水凝胶模型还可进一步应用于构建复杂的微图案结构，例如圆形、方形、十字形和环形等单个微凝胶，以及通过双环组装生成的包含成骨和血管化区域的骨样结构。这些结果为该黏附性大孔光交联水凝胶系统提供了作为指导组织形成的潜在三维支架，特别是用于具有多种细胞类型且需要精确定义细胞-细胞和细胞-基底相互作用的组织的生物工程。
