Shibata T, Burger P C, Kleihues P
Laboratory of Neuropathology, University of Zürich, Switzerland.
Acta Neurochir Suppl (Wien). 1988;43:103-6. doi: 10.1007/978-3-7091-8978-8_22.
The monoclonal antibody Ki-67 recognizes a nuclear antigen which is expressed during the G1, S, G2 and M phases of the cell cycle. Immunostaining of frozen biopsy material thus presents a convenient and rapid method for the estimation of the growth fraction in human neoplasia. This report summarizes the results obtained in 178 neurosurgical biopsies. The highest incidence of Ki-67 positive nuclei was observed in 8 metastatic carcinomas (mean: 20%) and in 53 cases of glioblastoma multiforme (10%). Glioblastomas showed considerable variation, the fraction of stained nuclei ranging from 1 to 28%. For astrocytomas and oligodendrogliomas we found a close correlation between the Ki-67 index and the histological grade in agreement with known biological behaviour. In anaplastic gliomas (WHO grade III) the upper limit was 11%, in grade II gliomas, 6%. Juvenile pilocytic astrocytomas and pituitary adenomas showed mean staining indices of approximately 1%. In 13 meningiomas the mean Ki-67 index was 1% but rose to 5% in recurrent and anaplastic meningiomas. Assessment of the growth fraction in human brain tumours by immunostaining with Ki-67 could become an important tool in the prediction of the biological behaviour of nervous system neoplasms and the planning of adjuvant therapy.
单克隆抗体Ki-67可识别一种核抗原，该抗原在细胞周期的G1、S、G2和M期表达。因此，对冷冻活检材料进行免疫染色为评估人类肿瘤的生长分数提供了一种便捷快速的方法。本报告总结了178例神经外科活检的结果。在8例转移性癌（平均：20%）和53例多形性胶质母细胞瘤（10%）中观察到Ki-67阳性细胞核的发生率最高。胶质母细胞瘤显示出相当大的差异，染色细胞核的比例为1%至28%。对于星形细胞瘤和少突胶质细胞瘤，我们发现Ki-67指数与组织学分级密切相关，这与已知的生物学行为一致。在间变性胶质瘤（世界卫生组织III级）中，上限为11%，在II级胶质瘤中为6%。青少年毛细胞型星形细胞瘤和垂体腺瘤的平均染色指数约为1%。在13例脑膜瘤中，平均Ki-67指数为1%，但在复发性和间变性脑膜瘤中升至5%。用Ki-67进行免疫染色评估人类脑肿瘤的生长分数可能成为预测神经系统肿瘤生物学行为和辅助治疗计划的重要工具。
