Austdal Marie, Skråstad Ragnhild Bergene, Gundersen Astrid Solberg, Austgulen Rigmor, Iversen Ann-Charlotte, Bathen Tone Frost
Department of Circulation and Medical Imaging, Faculty of Medicine, Norwegian University of Science and Technology (NTNU), Trondheim, Norway; Liaison Committee between the Central Norway Regional Health Authority (RHA) and Norwegian University of Science and Technology (NTNU), Trondheim, Norway; St. Olavs Hospital, Trondheim University Hospital, Trondheim, Norway.
Department of Laboratory Medicine, Children's and Women's Health, Faculty of Medicine, Norwegian University of Science and Technology (NTNU), Trondheim, Norway; National Center for Fetal Medicine, St. Olavs Hospital, Trondheim, Norway.
PLoS One. 2014 Mar 17;9(3):e91923. doi: 10.1371/journal.pone.0091923. eCollection 2014.
To explore the potential of magnetic resonance (MR) metabolomics for study of preeclampsia, for improved phenotyping and elucidating potential clues to etiology and pathogenesis.
Urine and serum samples from pregnant women with preeclampsia (n = 10), normal pregnancies (n = 10) and non-pregnant women (n = 10) matched by age and gestational age were analyzed with MR spectroscopy and subjected to multivariate analysis. Metabolites were then quantified and compared between groups.
Urine and serum samples revealed clear differences between women with preeclampsia and both control groups (normal pregnant and non-pregnant women). Nine urine metabolites were significantly different between preeclampsia and the normal pregnant group. Urine samples from women with early onset preeclampsia clustered together in the multivariate analysis. The preeclampsia serum spectra showed higher levels of low and very-low density lipoproteins and lower levels of high-density lipoproteins when compared to both non-pregnant and normal pregnant women.
The MR determined metabolic profiles in urine and serum from women with preeclampsia are clearly different from normal pregnant women. The observed differences represent a potential to examine mechanisms underlying different preeclampsia phenotypes in urine and serum samples in larger studies. In addition, similarities between preeclampsia and cardiovascular disease in metabolomics are demonstrated.
探讨磁共振（MR）代谢组学在子痫前期研究中的潜力，以改善表型分析并阐明病因和发病机制的潜在线索。
采用磁共振波谱分析对年龄和孕周匹配的子痫前期孕妇（n = 10）、正常孕妇（n = 10）和非孕妇（n = 10）的尿液和血清样本进行分析，并进行多变量分析。然后对各组间的代谢物进行定量和比较。
子痫前期孕妇的尿液和血清样本与两个对照组（正常孕妇和非孕妇）之间存在明显差异。子痫前期组与正常孕妇组之间有9种尿液代谢物存在显著差异。早发型子痫前期孕妇的尿液样本在多变量分析中聚集在一起。与非孕妇和正常孕妇相比，子痫前期孕妇的血清谱显示低密度脂蛋白和极低密度脂蛋白水平较高，而高密度脂蛋白水平较低。
磁共振测定的子痫前期孕妇尿液和血清中的代谢谱与正常孕妇明显不同。观察到的差异表明在更大规模的研究中，有潜力在尿液和血清样本中研究不同子痫前期表型的潜在机制。此外，还证明了子痫前期与心血管疾病在代谢组学方面的相似性。
