Menendez-Pelaez A, Reiter R J, Gonzalez-Brito A, Howes K A
Department of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio 78284-7762.
Arch Biochem Biophys. 1989 Jan;268(1):20-5. doi: 10.1016/0003-9861(89)90560-2.
The activities of N-acetyltransferase (NAT) and hydroxyindole-O-methyltransferase (HIOMT) and the indole contents of the Harderian glands of male Syrian hamsters were studied throughout a 24-h period. NAT activity exhibited a sharp rise 1 h after lights on, decreasing to basal levels 1 h later. Neither a HIOMT activity nor a melatonin concentration rhythm was detected throughout the 24 h. The 5-hydroxytryptamine (serotonin) concentration was highest during the dark phase reaching a peak at 0300 h; with light onset serotonin levels exhibited a rapid short-term drop. The 5-hydroxytryptophol concentration was highest during the mid- to late photophase; the lowest values to this constituent were measured late in the dark phase and at 1 h after lights on. The 5-hydroxyindole acetic acid concentration of the Harderian glands was rather stable throughout the 24-h period but levels did show a short-lived drop 1 h after light onset. Only a few animals contained detectable amounts of N-acetyl-5-hydroxytryptamine (N-acetylserotonin) in their Harderian glands. In agreement with previous work on the Harderian glands of female Syrian hamsters, the present results in males suggest that light onset is associated with marked changes in Harderian indoleamine metabolism.
在24小时期间，对雄性叙利亚仓鼠哈德氏腺的N-乙酰转移酶（NAT）和羟基吲哚-O-甲基转移酶（HIOMT）的活性以及吲哚含量进行了研究。NAT活性在光照后1小时急剧上升，1小时后降至基础水平。在整个24小时内均未检测到HIOMT活性和褪黑素浓度节律。5-羟色胺（血清素）浓度在黑暗期最高，在0300时达到峰值；随着光照开始，血清素水平迅速出现短期下降。5-羟色醇浓度在光照期中期至后期最高；该成分的最低值在黑暗期末期和光照后1小时测得。哈德氏腺的5-羟基吲哚乙酸浓度在整个24小时期间相当稳定，但在光照后1小时确实出现了短暂下降。只有少数动物的哈德氏腺中含有可检测量的N-乙酰-5-羟色胺（N-乙酰血清素）。与之前对雌性叙利亚仓鼠哈德氏腺的研究结果一致，目前对雄性仓鼠的研究结果表明，光照开始与哈德氏腺吲哚胺代谢的显著变化有关。
