Barazandeh Tehrani Maliheh, Ghanbari Hutan, Souri Effat, Shamsa Fazel, Amini Mohsen
Department of Medicinal Chemistry, Faculty of Pharmacy and Pharmaceutical Science Research Center, Tehran University of Medical Sciences, Tehran 1417614411, Iran.
Int J Anal Chem. 2014;2014:260179. doi: 10.1155/2014/260179. Epub 2014 Feb 4.
A simple, sensitive, accurate, and green spectrophotometric method for the determination of Cu(II) using newly synthesized reagent, 6-(2-methoxynaphthyl)-2,3-dihydro-1,2,4-triazine-3-thione (MNDTT), has been developed. MNDTT was synthesized based on the acylation of methoxy naphthalene and reaction of the product with amyl nitrite, which upon reaction with thiosemicarbazide yielded 6-(2-meyhoxynaphthyl)-2,3-dihydro-1,2,4-triazine-3-thione. MNDTT produces a dark red complex with copper in methanol according to the 1 : 2 stoichiometry. Beer's law was obeyed over the concentration range of 2.5-20 µg/mL with r (2) = 0.992. The limit of detection and limit of quantification were 0.33 and 1.10 µg/mL, respectively. Within-day and between-day precision values were less than 3.68%. Finally, the method has been applied to a dental alloy (110-plus) successfully and the results were compared with atomic absorption method. The results showed that there was no significant difference between the two methods (P > 0.05).
已开发出一种简单、灵敏、准确且绿色的分光光度法，用于使用新合成的试剂6-(2-甲氧基萘基)-2,3-二氢-1,2,4-三嗪-3-硫酮（MNDTT）测定铜（II）。MNDTT是基于甲氧基萘的酰化反应以及产物与亚硝酸戊酯的反应合成的，该产物与氨基硫脲反应生成6-(2-甲氧基萘基)-2,3-二氢-1,2,4-三嗪-3-硫酮。MNDTT在甲醇中与铜以1∶2的化学计量比生成深红色络合物。在2.5 - 20μg/mL的浓度范围内符合比尔定律，r² = 0.992。检测限和定量限分别为0.33和1.10μg/mL。日内和日间精密度值均小于3.68%。最后，该方法已成功应用于一种牙科合金（110-plus），并将结果与原子吸收法进行了比较。结果表明两种方法之间无显著差异（P > 0.05）。
