Ozel Rıfat Emrah, Wallace Kenneth N, Andreescu Silvana
Department of Chemistry and Biomolecular Science, Clarkson University, 8 Clarkson Ave. Potsdam, NY, 13699-5810, USA.
Department of Biology, Clarkson University, 8 Clarkson Ave. Potsdam, NY, 13699-5805, USA.
Environ Sci Nano. 2014 Feb 1;2014(1):27-36. doi: 10.1039/C3EN00001J.
The increased use of engineered nanoparticles (NPs) in manufacturing and consumer products raises concerns about the potential environmental and health implications on the ecosystem and living organisms. Organs initially and more heavily affected by environmental NPs exposure in whole organisms are the skin and digestive system. We investigate the toxic effect of two types of NPs, nickel (Ni) and copper oxide (CuO), on the physiology of the intestine of a living aquatic system, zebrafish embryos. Embryos were exposed to a range of Ni and CuO NP concentrations at different stages of embryonic development. We use changes in the physiological serotonin (5HT) concentrations, determined electrochemically with carbon fiber microelectrodes inserted in the live embryo, to assess this organ dysfunction due to NP exposure. We find that exposure to both Ni and CuO NPs induces changes in the physiological 5HT concentration that varies with the type, exposure period and concentration of NPs, as well as with the developmental stage during which the embryo is exposed. These data suggest that exposure to NPs might alter development and physiological processes in living organisms and provide evidence of the effect of NPs on the physiology of the intestine.
工程纳米颗粒（NPs）在制造和消费品中的使用增加，引发了人们对其对生态系统和生物体潜在的环境及健康影响的担忧。在整个生物体中，最初且受环境纳米颗粒暴露影响更严重的器官是皮肤和消化系统。我们研究了两种纳米颗粒，镍（Ni）和氧化铜（CuO），对水生生物系统斑马鱼胚胎肠道生理的毒性作用。在胚胎发育的不同阶段，将胚胎暴露于一系列镍和氧化铜纳米颗粒浓度下。我们使用插入活胚胎中的碳纤维微电极通过电化学方法测定生理血清素（5HT）浓度的变化，以评估由于纳米颗粒暴露导致的该器官功能障碍。我们发现，暴露于镍和氧化铜纳米颗粒都会引起生理5HT浓度的变化，这种变化随纳米颗粒的类型、暴露时间、浓度以及胚胎暴露时的发育阶段而有所不同。这些数据表明，暴露于纳米颗粒可能会改变生物体的发育和生理过程，并为纳米颗粒对肠道生理的影响提供了证据。
