Vasin A V, Sandybaev N T, Plotnikova M A, Klotchenko S A, Cherviakova O V, Strochkov V M, Taĭlakova É T, Temkina O A, Brodskaia A V, Zabrodskaia Ia A, Nikulenkov K P, Egorov V V, Koshemetov Zh K, Sancyzbaĭ A R, Kiselev O I
Vopr Virusol. 2013 Sep-Oct;58(5):32-7.
The diagnostic oligonucleotide microarray for subtyping of human and animal influenza A viruses (IAVs) was developed. We proposed a simple method of the fluorescent labeling of genomic segments of all known IAVs subtypes, the composition of the hybridization buffer, as well as the software of the data processing. 48 IAVs strains of different subtypes were analyzed using our microarray. All of them were identified, while 45 of 48 strains were unambiguously subtyped.
开发了用于人类和动物甲型流感病毒（IAV）亚型分型的诊断性寡核苷酸微阵列。我们提出了一种对所有已知IAV亚型的基因组片段进行荧光标记的简单方法、杂交缓冲液的组成以及数据处理软件。使用我们的微阵列分析了48株不同亚型的IAV。所有毒株均被鉴定出来，48株中的45株被明确分型。
