Sultankulova K T, Chervyakova O V, Kozhabergenov N S, Shorayeva K A, Strochkov V M, Orynbayev M B, Sandybayev N T, Sansyzbay A R, Vasin A V
Research Institute for Biological Safety Problems (RIBSP), Science Committee of Ministry of Education and Science of Republic of Kazakhstan, Gvardeiskiy, Kordaiskiy Rayon, Zhambylskaya Oblast 080409, Kazakhstan.
Research Institute of Influenza, Ministry of Healthcare of the Russian Federation, Prof. Popov Street 15/17, Saint Petersburg 197376, Russia.
ScientificWorldJournal. 2014;2014:620580. doi: 10.1155/2014/620580. Epub 2014 Nov 23.
The paper describes comparative evaluation of IAVchip DNA microarray, reverse transcription PCR (RT-PCR), and real-time RT-PCR versus virus isolation in chicken embryos and shows their diagnostic effectiveness in detection and subtyping of influenza A virus. The tests were evaluated with use of 185 specimens from humans, animals, and birds. IAVchip DNA microarray demonstrates higher diagnostic effectiveness (99.45%) in early influenza A diagnosis as compared to the real-time PCR (98.38%) and RT-PCR (96.22%), thus showing its clear superiority. Diagnostic sensitivity of IAVchip DNA microarray (100%) exceeds the same of RT-PCR (95.95%) and real-time RT-PCR (97.96%) in the range of estimated confidence intervals. IAVchip DNA microarray and real-time RT-PCR displayed equal diagnostic specificity (98.85%), while diagnostic specificity of RT-PCR was 96.40%. IAVchip DNA microarray has an advantage over the other tests for influenza A diagnosis and virus identification as a more rapid method that allows performing simultaneous detection and subtyping of about tens of specimens within one experiment during 8-10 hours. The developed IAVchip DNA microarray is a general test tool that enables identifying simultaneously 16 hemagglutinin (HA) and 9 neuraminidase (NA) subtypes of influenza A virus and also to screen the influenza A viruses from humans, animals, and birds by M and NP genes.
本文描述了IAVchip DNA微阵列、逆转录PCR（RT-PCR）和实时RT-PCR与鸡胚病毒分离法在检测甲型流感病毒方面的比较评估，并展示了它们在甲型流感病毒检测和亚型鉴定中的诊断效力。使用来自人类、动物和鸟类的185份标本对这些检测方法进行了评估。与实时PCR（98.38%）和RT-PCR（96.22%）相比，IAVchip DNA微阵列在甲型流感早期诊断中显示出更高的诊断效力（99.45%），从而表明其明显优势。在估计的置信区间范围内，IAVchip DNA微阵列的诊断敏感性（100%）超过RT-PCR（95.95%）和实时RT-PCR（97.96%）。IAVchip DNA微阵列和实时RT-PCR显示出相同的诊断特异性（98.85%），而RT-PCR的诊断特异性为96.40%。IAVchip DNA微阵列作为一种更快速的方法，在甲型流感诊断和病毒鉴定方面优于其他检测方法，它能够在8至10小时内的一次实验中同时检测和约数十份标本的亚型。所开发的IAVchip DNA微阵列是一种通用检测工具，能够同时鉴定甲型流感病毒的16种血凝素（HA）和9种神经氨酸酶（NA）亚型，还能通过M基因和NP基因从人类、动物和鸟类中筛选甲型流感病毒。
