Han Xueqing, Lin Xiangmei, Liu Bohua, Hou Yihong, Huang Jinhai, Wu Shaoqiang, Liu Jian, Mei Lin, Jia Guangle, Zhu Qingyu
Chinese Academy of Inspection and Quarantine, Beijing 100029, China.
J Virol Methods. 2008 Sep;152(1-2):117-21. doi: 10.1016/j.jviromet.2008.05.027. Epub 2008 Jul 18.
Rapid diagnosis of novel emerging subtypes of influenza viruses is vital for effective global influenza surveillance. To this end, a novel microarray based surveillance was developed for subtyping all influenza A viruses on one chip. Using reference strains of different influenza subtypes and samples from different areas, the results show that all the subtypes of the influenza A virus could be identified simultaneously on this microchip with high sensitivity. There was no cross-hybridization reaction with other viruses, indicating that the microarray is specific for influenza A viruses. Such a diagnostic microarray will undoubtedly be useful for identifying novel influenza A virus subtypes.
快速诊断新出现的新型流感病毒亚型对于有效的全球流感监测至关重要。为此，开发了一种基于微阵列的新型监测方法，用于在一个芯片上对所有甲型流感病毒进行亚型分型。使用不同流感亚型的参考菌株和来自不同地区的样本，结果表明，该微芯片能够同时以高灵敏度鉴定甲型流感病毒的所有亚型。与其他病毒没有交叉杂交反应，这表明该微阵列对甲型流感病毒具有特异性。这种诊断微阵列无疑将有助于鉴定新型甲型流感病毒亚型。
