纳米大黄素传递体的制备及其对饮食诱导肥胖大鼠脂肪组织抗肥胖机制的研究
Preparation of a nano emodin transfersome and study on its anti-obesity mechanism in adipose tissue of diet-induced obese rats.
作者信息
Lu Kun, Xie Shuanshuan, Han Shilong, Zhang Jidong, Chang Xinwen, Chao Jin, Huang Qingqing, Yuan Qing, Lin Haiyan, Xu Lei, Shen Changxing, Tan Min, Qu Shen, Wang Changhui, Song Xiaolian
机构信息
Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, China.
出版信息
J Transl Med. 2014 Mar 19;12:72. doi: 10.1186/1479-5876-12-72.
OBJECTIVE
To describe the preparation of nano emodin transfersome (NET) and investigate its effect on mRNA expression of adipose triglyceride lipase (ATGL) and G0/G1 switch gene 2 (G0S2) in adipose tissue of diet-induced obese rats.
METHODS
NET was prepared by film-ultrasonic dispersion method. The effects of emodin components at different ratios on encapsulation efficiency were investigated.The NET envelopment rate was determined by ultraviolet spectrophotometry. The particle size and Zeta potential of NET were evaluated by Zetasizer analyzer. Sixty male SD rats were assigned to groups randomly. After 8-week treatment, body weight, wet weight of visceral fat and the percentage of body fat (PBF) were measured. Fasting blood glucose and serum lipid levels were determined. The adipose tissue section was HE stained, and the cellular diameter and quantity of adipocytes were evaluated by light microscopy. The mRNA expression of ATGL and G0S2 from the peri-renal fat tissue was assayed by RT-PCR.
RESULTS
The appropriate formulation was deoxycholic acid sodium salt vs. phospholipids 1:8, cholesterol vs. phospholipids 1:3, vitamin Evs. phospholipids 1:20, and emodin vs. phospholipid 1:6. Zeta potential was -15.11 mV, and the particle size was 292.2 nm. The mean encapsulation efficiency was (69.35 ± 0.25)%. Compared with the obese model group, body weight, wet weight of visceral fat, PBF and mRNA expression of G0S2 from peri-renal fat tissue were decreased significantly after NET treatment (all P < 0.05), while high-density lipoprotein cholesterol (HDL-C), the diameter of adipocytes and mRNA expression of ATGL from peri-renal fat tissue were increased significantly (all P < 0.05).
CONCLUSION
The preparation method is simple and reasonable. NET with negative electricity was small and uniform in particle size, with high encapsulation efficiency and stability. NET could reduce body weight and adipocyte size, and this effect was associated with the up-regulation of ATGL, down-regulation of G0S2 expression in the adipose tissue, and improved insulin sensitivity.
目的
描述纳米大黄素传递体（NET）的制备方法，并研究其对饮食诱导肥胖大鼠脂肪组织中脂肪甘油三酯脂肪酶（ATGL）和G0/G1转换基因2（G0S2）mRNA表达的影响。
方法
采用薄膜超声分散法制备NET。研究不同比例大黄素成分对包封率的影响。通过紫外分光光度法测定NET的包封率。用Zetasizer分析仪评估NET的粒径和Zeta电位。将60只雄性SD大鼠随机分组。治疗8周后，测量体重、内脏脂肪湿重和体脂百分比（PBF）。测定空腹血糖和血脂水平。对脂肪组织切片进行HE染色，通过光学显微镜评估脂肪细胞的直径和数量。采用RT-PCR法检测肾周脂肪组织中ATGL和G0S2的mRNA表达。
结果
合适的配方为脱氧胆酸钠与磷脂比例为1:8，胆固醇与磷脂比例为1:3，维生素E与磷脂比例为1:20，大黄素与磷脂比例为1:6。Zeta电位为-15.11 mV，粒径为292.2 nm。平均包封率为（69.35±0.25）%。与肥胖模型组相比，NET治疗后体重、内脏脂肪湿重、PBF及肾周脂肪组织中G0S2的mRNA表达均显著降低（均P<0.05），而高密度脂蛋白胆固醇（HDL-C）、脂肪细胞直径及肾周脂肪组织中ATGL的mRNA表达均显著升高（均P<0.05）。
结论
该制备方法简单合理。带负电的NET粒径小且均匀，包封率高，稳定性好。NET可减轻体重、减小脂肪细胞大小，且这种作用与脂肪组织中ATGL上调、G0S2表达下调及胰岛素敏感性改善有关。
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