Szymanski Jedrzej, Brotman Yariv, Willmitzer Lothar, Cuadros-Inostroza Álvaro
Max Planck Institute of Molecular Plant Physiology, 14476 Potsdam-Golm, Germany.
Plant Cell. 2014 Mar;26(3):915-28. doi: 10.1105/tpc.113.118919. Epub 2014 Mar 18.
Glycerolipid metabolism of plants responds dynamically to changes in light intensity and temperature, leading to the modification of membrane lipid composition to ensure optimal biochemical and physical properties in the new environment. Although multiple posttranscriptional regulatory mechanisms have been reported to be involved in the process, the contribution of transcriptional regulation remains largely unknown. Here, we present an integrative analysis of transcriptomic and lipidomic data, revealing large-scale coordination between gene expression and changes in glycerolipid levels during the Arabidopsis thaliana response to light and temperature stimuli. Using a multivariate regression technique called O2PLS, we show that the gene expression response is strictly coordinated at the biochemical pathway level and occurs in parallel with changes of specific glycerolipid pools. Five interesting candidate genes were chosen for further analysis from a larger set of candidates identified based on their close association with various groups of glycerolipids. Lipidomic analysis of knockout mutant lines of these five genes showed a significant relationship between the coordination of transcripts and glycerolipid levels in a changing environment and the effects of single gene perturbations.
植物的甘油脂质代谢对光照强度和温度的变化会做出动态响应，从而导致膜脂成分的改变，以确保在新环境中具备最佳的生化和物理特性。尽管已有报道称多种转录后调控机制参与了这一过程，但转录调控的作用仍 largely 未知。在此，我们对转录组学和脂质组学数据进行了综合分析，揭示了拟南芥对光和温度刺激响应过程中基因表达与甘油脂质水平变化之间的大规模协同作用。使用一种名为 O2PLS 的多元回归技术，我们表明基因表达响应在生化途径水平上是严格协调的，并且与特定甘油脂质库的变化同时发生。从基于与各类甘油脂质密切关联而鉴定出的大量候选基因中，挑选了五个有趣的候选基因进行进一步分析。对这五个基因的敲除突变体系进行脂质组学分析，结果表明在变化的环境中，转录本与甘油脂质水平的协同作用以及单基因扰动的影响之间存在显著关系。
