Horváth Györgyi, Acs Kamilla, Kocsis Béla
J AOAC Int. 2013 Nov-Dec;96(6):1209-13. doi: 10.5740/jaoacint.sgehorvath.
The aim of the present study was the chemical characterization of the essential oil of a Mongolian medicinal plant, Artemisia adamsii Besser, and the investigation of the antibacterial effect of its oil on different human pathogenic bacteria (Staphylococcus aureus, methicillin-resistant S. aureus, and S. epidermidis). The chemical composition of the oil was established by GC and GC/MS. Direct bioautography was used for detecting the antibacterial activity of the essential oil. The result of GC experiments showed that a-thujone was the main component (64.4%) of the oil, while the amount of beta-thujone was 7.1%. 1,8-Cineole seemed to be the other relevant component (15.2%). The antibacterial activity of the A. adamsii essential oil against all three investigated bacteria was observed in the bioautographic system, but this effect was not proportional to the concentrations of a- or beta-thujone; therefore, from a microbiological aspect, thujone content does not determine the medicinal value of this oil. On the whole, the combination of TLC separation with biological detection is an appropriate method for evaluating multicomponent and hydrophobic plant extracts, for instance, essential oils, and it provides more reliable results than traditional microbiological methods (e.g., disc diffusion and agar plate techniques).
本研究的目的是对蒙古药用植物中亚滨藜（Artemisia adamsii Besser）的精油进行化学表征，并研究其精油对不同人类病原菌（金黄色葡萄球菌、耐甲氧西林金黄色葡萄球菌和表皮葡萄球菌）的抗菌作用。通过气相色谱（GC）和气相色谱-质谱联用（GC/MS）确定了该精油的化学成分。采用直接生物自显影法检测精油的抗菌活性。GC实验结果表明，α-侧柏酮是该精油的主要成分（64.4%），而β-侧柏酮的含量为7.1%。1,8-桉叶素似乎是另一种相关成分（15.2%）。在生物自显影系统中观察到中亚滨藜精油对所有三种受试细菌均有抗菌活性，但这种作用与α-或β-侧柏酮的浓度不成正比；因此，从微生物学角度来看，侧柏酮含量并不能决定该精油的药用价值。总体而言，薄层色谱分离与生物检测相结合是评估多成分和疏水性植物提取物（如精油）的合适方法，它比传统微生物学方法（如纸片扩散法和琼脂平板技术）能提供更可靠的结果。
