采用分析和统计方法相结合，以优化快乐鼠尾草超临界流体提取物的抗菌活性。

Combination of Analytical and Statistical Methods in Order to Optimize Antibacterial Activity of Clary Sage Supercritical Fluid Extracts.

机构信息

Department of Pharmacognosy, Faculty of Pharmacy, University of Pécs, H-7624 Pécs, Hungary.

Institute of Chemical Engineering and Process Engineering, Faculty of Engineering, University of Pannonia, H-8200 Veszprém, Hungary.

出版信息

Molecules. 2021 Oct 26;26(21):6449. doi: 10.3390/molecules26216449.

The extraction of clary sage ( L.) using supercritical carbon dioxide (SC-CO) was systematically studied by using thin layer chromatography-direct bioautography (TLC-DB) and response surface methodology (RSM). The three parameters temperature, pressure, and cosolvent ratio were optimized for the maximum antibacterial activity of clary sage extracts against () and methicillin-resistant (MRSA). The highest inhibition zone was 7.51 mm for and 7.57 mm for MRSA. According to RSM analysis, the predicted optimum extraction parameters are 18.6 MPa pressure, 40 °C temperature, and 2% ethanol (EtOH) ratio. The combination of this analytical and statistical method allows saving time, money, and instrument runtime in the optimization of essential oil composition, which is tailored to a specific task and could be useful on any kind of herbs in a wide range of use from perfume manufacturing to the food industry.

利用薄层色谱-直接生物自显影（TLC-DB）和响应面法（RSM）系统地研究了用超临界二氧化碳（SC-CO2）萃取快乐鼠尾草（L.）。优化了温度、压力和共溶剂比这三个参数，以获得对（）和耐甲氧西林金黄色葡萄球菌（MRSA）的最高抗菌活性的快乐鼠尾草提取物。对和 MRSA 的最高抑菌圈分别为 7.51mm 和 7.57mm。根据 RSM 分析，预测的最佳提取参数为 18.6MPa 压力、40°C 温度和 2%乙醇（EtOH）比例。这种分析和统计方法的结合可以节省时间、金钱和仪器运行时间，从而优化特定任务所需的精油成分，并且可以在从香水制造到食品工业等广泛用途的任何种类的草药中使用。