Manzo Alessandra, Musso Loana, Panseri Sara, Iriti Marcello, Dallavalle Sabrina, Catalano Enrico, Scarì Giorgio, Giorgi Annamaria
Centre for Applied Studies in the Sustainable Management and Protection of the Mountain Environment-Ge.S.Di.Mont, University of Milan, Via Morino 8, 25048, Edolo, Brescia, Italy.
Department of Food, Environmental and Nutritional Sciences, University of Milan, Via Celoria 2, 20133, Milan, Italy.
J Sci Food Agric. 2016 Jul;96(9):3195-201. doi: 10.1002/jsfa.7499. Epub 2015 Nov 11.
This research aimed at improving knowledge as to the chemical composition and the antibacterial and anti-cancer activities of the essential oil of Waldheimia glabra, a wild plant from the Himalayan Mountains.
The results obtained by GC-MS showed that spathulenol, 9-tetradecenol, thujopsene, α-thujone, santolina alcohol and terpinen-4-ol were the main constituents of Waldheimia glabra essential oil. These results were confirmed by HS-SPME GC-MS analysis that also reported high amounts of artemisia alcohol and camphor. Disc diffusion assay suggested a mild antibacterial activity against both Escherichia coli and Staphylococcus aureus. Finally, a dose-response correlation was observed between Waldhemia glabra essential oil concentration and viability of human breast adenocarcinoma cells MDA-MB-231 and MCF-7.
Together with the GC-MS method, HS-SPME GC-MS proved to be a reliable technique to characterise the chemical composition of essential oil obtained from aromatic plants. Further studies will focus on W. glabra phytochemicals and their biological activity, in order to support traditional uses of the plant. © 2015 Society of Chemical Industry.
本研究旨在增进对喜马拉雅山脉野生植物光叶委陵菜精油化学成分及其抗菌和抗癌活性的了解。
气相色谱 - 质谱联用（GC - MS）分析结果表明，斯巴醇、9 - 十四碳烯醇、罗汉柏烯、α - 侧柏酮、山道年醇和萜品烯 - 4 - 醇是光叶委陵菜精油的主要成分。顶空固相微萃取 - 气相色谱 - 质谱联用（HS - SPME GC - MS）分析也证实了这些结果，该分析还报告了大量的蒿醇和樟脑。纸片扩散法表明其对大肠杆菌和金黄色葡萄球菌均有轻度抗菌活性。最后，观察到光叶委陵菜精油浓度与人类乳腺腺癌细胞MDA - MB - 231和MCF - 7的活力之间存在剂量反应相关性。
与GC - MS方法一起，HS - SPME GC - MS被证明是一种可靠的技术，可用于表征从芳香植物中获得的精油的化学成分。进一步的研究将集中在光叶委陵菜的植物化学物质及其生物活性上，以支持该植物的传统用途。© 2015化学工业协会。
