冷大气等离子体、氢氧化钙和三联抗生素糊剂对根管系统生物膜抗菌作用的评估：一项体外研究。
Evaluation of Antibacterial Effects of Cold Atmospheric Plasma, Calcium Hydroxide, and Triple Antibiotic Paste on Biofilm in the Root Canal System: An In Vitro Study.
作者信息
Asnaashari Mohammad, Mehrabinia Pegah, Yadegari Zahra, Hoseini Hanieh, Sadafi Mehdi, Shojaeian Shiva
机构信息
Laser Application in Medical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Post Graduate Student, Department of Endodontics, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
出版信息
J Lasers Med Sci. 2022 Nov 21;13:e50. doi: 10.34172/jlms.2022.50. eCollection 2022.
One of the essential factors in successful endodontic therapy is the effective cleaning and disinfection of the root canal. This study aimed to determine the effect of cold plasma on infected root canals with and compare its antibacterial effect with the conventional medicaments in vitro. Sixty-tree single-root teeth were extracted. Canals were cleaned and shaped. Ten teeth were selected as the negative control randomly. The rest of the teeth were incubated at 37°C for 21 days to form biofilm. The specimens were divided into six groups; each group had 10 teeth. In group 1 (the positive control group of calcium hydroxide and triple antibiotic paste [TAP]), methylcellulose was placed in the root canal; in group 2, calcium hydroxide was placed in the root canal for 12 days; in group 3, 10 mg/mL of TAP was placed in the root canal for 12 days; in group 4, helium/oxygen plasma jet was used for 10 minutes. Group 5 was considered as a positive control of plasma, and group 6 was the negative control. After treatment, F4 Pro-Taper rotary file was used to collect root canal microbial biofilms. Bacterial suspensions were serially diluted, and the percentage of growth reduction for each group was obtained by dividing the logarithm of CFU/mL of each group by CFU/mL of the control of the same group. The CFU/mL of TAP and plasma-treated samples was significantly lower than that of the control groups; however, there were no significant differences between the control group and the samples treated by calcium hydroxide. The most percentage of CFU reduction was in the TAP-treated group compared with plasma and calcium hydroxide-treated groups. The application of cold plasma effectively inhibited the growth of and reduced bacterial biofilm. Also, in the present study, 10 mg/mL of TAP caused the complete elimination of . Calcium hydroxide had the most negligible effect on biofilm elimination.
成功进行牙髓治疗的关键因素之一是对根管进行有效的清洁和消毒。本研究旨在确定冷等离子体对感染根管的作用，并在体外将其抗菌效果与传统药物进行比较。提取了63颗单根牙。对根管进行清理和塑形。随机选择10颗牙齿作为阴性对照。其余牙齿在37°C下孵育21天以形成生物膜。将标本分为六组；每组有10颗牙齿。在第1组（氢氧化钙和三联抗生素糊剂[TAP]阳性对照组）中，将甲基纤维素置于根管内；在第2组中，将氢氧化钙置于根管内12天；在第3组中，将10mg/mL的TAP置于根管内12天；在第4组中，使用氦/氧等离子体射流处理10分钟。第5组被视为等离子体阳性对照，第6组为阴性对照。治疗后，使用F4 Pro-Taper旋转锉收集根管微生物生物膜。将细菌悬液进行系列稀释，通过将每组CFU/mL的对数除以同组对照的CFU/mL来获得每组生长减少的百分比。TAP和等离子体处理样本的CFU/mL显著低于对照组；然而，对照组与氢氧化钙处理样本之间没有显著差异。与等离子体和氢氧化钙处理组相比，TAP处理组的CFU减少百分比最高。冷等离子体的应用有效地抑制了[细菌名称未明确]的生长并减少了细菌生物膜。此外，在本研究中，10mg/mL的TAP导致[细菌名称未明确]完全消除。氢氧化钙对生物膜消除的影响最小。
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