Rastegar Khosravi Mohammad, Khonsha Masoumeh, Ramazanzadeh Rashid
Department of Endodontics, Faculty of Dentistry, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Department of Microbiology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Iran Endod J. 2019 Winter;14(1):40-46. doi: 10.22037/iej.v14i1.21245.
Endodontic treatment of necrotic immature teeth poses several clinical challenges. A major problem is the elimination of microorganisms from the root canal system. This study evaluates the antibacterial efficacy of ciprofloxacin (CIP), levofloxacin (LEV), and their combination with N-acetylcysteine (NAC) in root canals infected with .
A total of 120 human extracted teeth with single canals were prepared and randomly divided into six groups: Calcium hydroxide (CH), ciprofloxacin (CIP), levofloxacin (LEV), ciprofloxacin and N-acetylcysteine (CIP+NAC), levofloxacin and N-acetylcysteine (LEV+NAC), and normal saline as a positive control. According to the name of the groups, intracanal medicaments were placed into the canals and the teeth were restored with a temporary filling. After one week, intracanal medicament was removed and the final count of bacteria was measured. Antibacterial effect of medicament was assessed by measuring the percentage reduction in the colony counts (RCC) and scanning electron microscopy (SEM). The Mann-Whitney U test and the Kruskal-Wallis test were used to compare the overall antibacterial efficacy of the intracanal medicaments at significance level of 0.05.
All intracanal medicaments were significantly more effective than calcium hydroxide (<0.05). The combination of LEV and NAC caused significantly higher reduction in colony count in comparison with other tested medicaments (=0.001).
The combination of LEV and NAC showed greater antibacterial activity compared with other tested medicaments against biofilm of Thus, it has the potential to be used in regenerative endodontic treatments.
对根尖未发育完成的坏死牙齿进行根管治疗面临着多项临床挑战。一个主要问题是从根管系统中清除微生物。本研究评估环丙沙星（CIP）、左氧氟沙星（LEV）及其与N - 乙酰半胱氨酸（NAC）联合使用对感染根管的抗菌效果。
共准备120颗单根管的人离体牙，并随机分为六组：氢氧化钙（CH）、环丙沙星（CIP）、左氧氟沙星（LEV）、环丙沙星与N - 乙酰半胱氨酸（CIP + NAC）、左氧氟沙星与N - 乙酰半胱氨酸（LEV + NAC），以及作为阳性对照的生理盐水。根据分组名称，将根管内药物放入根管，并用临时充填材料对牙齿进行修复。一周后，取出根管内药物并测量细菌的最终数量。通过测量菌落计数减少百分比（RCC）和扫描电子显微镜（SEM）评估药物的抗菌效果。采用Mann - Whitney U检验和Kruskal - Wallis检验，在显著性水平为0.05时比较根管内药物的总体抗菌效果。
所有根管内药物均比氢氧化钙显著更有效（<0.05）。与其他受试药物相比，LEV和NAC的组合导致菌落计数显著降低（=0.001）。
与其他受试药物相比，LEV和NAC的组合对[具体细菌名称未给出]生物膜显示出更大的抗菌活性。因此，它有潜力用于牙髓再生治疗。
