Schulz T F, Vogetseder W, Mitterer M, Neumayer H P, Myones B L, Stauder R, Greil R, Böck G, Feichtinger H, Huber H
Institute for Hygiene, Innsbruck, Austria.
Mol Immunol. 1988 Nov;25(11):1053-61. doi: 10.1016/0161-5890(88)90137-x.
The membrane molecule termed "7F7-antigen" has been found to be involved in several examples of cell-cell interactions. This 85 kDa glycoprotein with a protein core of about 55 kDa contains N-linked and O-linked carbohydrates. It has an isoelectric point of 8.0-8.5 and is expressed on 20% of peripheral blood mononuclear cells, 35% of peripheral blood B-cells, follicular dendritic cells and vascular endothelium. It is also expressed on activated T-cells and its expression on B-cells, fibroblasts and monocytes increases after treatment with PWM, interferon-gamma and after three days culture, respectively. The MAb 7F7 used to define this antigen inhibits the initiation of T-cell proliferation induced by anti-CD3, PHA, ConA and (weakly) allogenic stimulator cells, but does not affect the growth of IL-2 dependent T-cells and does not interfere with the killing of PHA-blasts by allogenic IL-2 dependent T-cells. 7F7 also inhibits the binding of C3-coated sheep erythrocytes to B-cells, the PMA-induced aggregation of U937 and the binding of activated T-cells to fibroblasts. The 7F7-antigen is expressed on some non-Hodgkin lymphomas of B-cell differentiation, particularly those with follicular structure, but not on Burkitt's lymphoma, ALL or carcinomas of various tissues. It is, however, found on fibrous tissue surrounding infiltrating carcinoma cells. The expression of a melanoma antigen, P3.58, which was shown to be identical to 7F7-antigen correlates with stage and spread of invasive melanoma. It was concluded that the 7F7-antigen, which is probably related to a previously described adherence molecule (ICAM-1), is of biological importance for the initiation of T-cell responses. With the possible exception of melanoma its expression on neoplastic cells in vivo is unlikely to be of importance for the spread of malignant disease.
一种被称为“7F7抗原”的膜分子已被发现参与了多个细胞间相互作用的实例。这种85千道尔顿的糖蛋白，其蛋白质核心约为55千道尔顿，含有N-连接和O-连接的碳水化合物。它的等电点为8.0 - 8.5，在外周血单核细胞的20%、外周血B细胞的35%、滤泡树突状细胞和血管内皮细胞上表达。它也在活化的T细胞上表达，在用PWM、干扰素-γ处理后以及分别在培养三天后，其在B细胞、成纤维细胞和单核细胞上的表达会增加。用于定义该抗原的单克隆抗体7F7可抑制由抗CD3、PHA、ConA和（较弱地）同种异体刺激细胞诱导的T细胞增殖的起始，但不影响IL-2依赖性T细胞的生长，也不干扰同种异体IL-2依赖性T细胞对PHA母细胞的杀伤。7F7还可抑制C3包被的绵羊红细胞与B细胞的结合、PMA诱导的U937聚集以及活化的T细胞与成纤维细胞的结合。7F7抗原在一些B细胞分化的非霍奇金淋巴瘤中表达，特别是那些具有滤泡结构的淋巴瘤，但在伯基特淋巴瘤、急性淋巴细胞白血病或各种组织的癌中不表达。然而，在浸润癌细胞周围的纤维组织中可以发现它。一种黑色素瘤抗原P3.58的表达，已证明其与7F7抗原相同，与侵袭性黑色素瘤的分期和扩散相关。得出的结论是，7F7抗原可能与先前描述的黏附分子（ICAM-1）相关，对于T细胞反应的起始具有生物学重要性。除黑色素瘤外，其在体内肿瘤细胞上的表达对于恶性疾病的扩散可能不太重要。
