Vogetseder W, Feichtinger H, Schulz T F, Schwaeble W, Tabaczewski P, Mitterer M, Böck G, Marth C, Dapunt O, Mikuz G
Institute for Hygiene, University of Innsbruck, Austria.
Int J Cancer. 1989 May 15;43(5):768-73. doi: 10.1002/ijc.2910430504.
The 7F7-antigen is a widespread 85-kDa membrane adherence molecule involved in heterotypic adhesion between PHA-blasts and fibroblasts. Immunofluorescence analysis of COS cells transfected with clone pICAM-I indicated that 7F7-antigen is identical with ICAM-I, the ligand for the contact molecule LFA-I. We have investigated the expression of ICAM-I on several human carcinomas. Tumor cells in most carcinomas, with the exception of squamous-cell carcinomas, expressed very little ICAM-I or none at all. In contrast, marked expression of this molecule was observed on fibrous tissue in the vicinity of carcinoma cells, its intensity correlating with lymphatic infiltration in these tumors. We also examined the expression of ICAM-I on carcinoma cell lines and its induction by treatment with interferon-gamma (IFN-gamma). The inducibility of ICAM-I expression on cultured fibroblasts by several lymphokines suggests that the expression of ICAM-I in the vicinity of carcinoma cells is effected by lymphokines produced by activated lymphocytes/macrophages within the tumor.
7F7抗原是一种广泛存在的85kDa膜黏附分子，参与PHA母细胞与成纤维细胞之间的异型黏附。对用克隆pICAM-1转染的COS细胞进行免疫荧光分析表明，7F7抗原与ICAM-1相同，ICAM-1是接触分子LFA-1的配体。我们研究了ICAM-1在几种人类癌组织中的表达情况。大多数癌组织中的肿瘤细胞，除了鳞状细胞癌外，表达很少的ICAM-1或根本不表达。相反，在癌细胞附近的纤维组织上观察到该分子的显著表达，其强度与这些肿瘤中的淋巴浸润相关。我们还检测了ICAM-1在癌细胞系中的表达及其受干扰素-γ（IFN-γ）处理后的诱导情况。几种淋巴因子对培养的成纤维细胞ICAM-1表达的诱导作用表明，癌细胞附近ICAM-1的表达是由肿瘤内活化淋巴细胞/巨噬细胞产生的淋巴因子所介导的。
