Bugaisky L B, Zak R
University of Alabama, Department of Pathology, Birmingham 35294.
Circ Res. 1989 Mar;64(3):493-500. doi: 10.1161/01.res.64.3.493.
Cardiac myocytes isolated from adult rat hearts were grown on laminin coated culture dishes for more than a month. During this time, the cells underwent a morphological transformation which has also been referred to by others as cell remodeling (Guo J-X, Jacobson SL, Brown DL: Cell Mot Cytoskeleton 1986;6:291-304). This results in a change in myocyte morphology from its typical in vivo cylindrical shape to one which is more pleiomorphic. Despite the long-term change in morphology, myocytes expressed for differing lengths of time several aspects of the adult phenotype as evidenced by the following: 1) maintenance of cylindrical shape and/or evident cross-striations for the first 24-48 hours in culture, 2) reappearance of cross-striations during the second week in culture, 3) little or no spontaneous contractility for the first 4 days in culture, 4) expression of only the V1 isoform of myosin for at least 7 days, and 5) altered myosin isoform expression in response to changes in environmental conditions. These factors taken together suggest that in culture the adult cardiac myocyte remains a highly differentiated cell (as opposed to possible dedifferentiation) and maintains many of its previous in vivo characteristics. Such highly differentiated adult cells should be suitable as an in vitro system for studying the direct cellular effects of factors which regulate growth and differentiation of the in vivo heart.
从成年大鼠心脏分离出的心肌细胞在包被有层粘连蛋白的培养皿上培养了一个多月。在此期间，细胞经历了形态转变，其他人也将其称为细胞重塑（郭J-X、雅各布森SL、布朗DL：《细胞运动与细胞骨架》1986年；6：291-304）。这导致心肌细胞形态从其典型的体内圆柱形变为更具多形性的形态。尽管形态发生了长期变化，但心肌细胞在不同时间段表达了成年表型的几个方面，如下所示：1）培养的前24-48小时保持圆柱形和/或明显的横纹，2）培养第二周横纹重新出现，3）培养的前4天很少或没有自发收缩性，4）至少7天仅表达肌球蛋白的V1同工型，5）响应环境条件变化而改变肌球蛋白同工型表达。综合这些因素表明，在培养中成年心肌细胞仍然是高度分化的细胞（与可能的去分化相反），并维持其许多先前的体内特征。这种高度分化的成年细胞应该适合作为体外系统，用于研究调节体内心脏生长和分化的因子的直接细胞效应。
