Stanfield B B, Trice J E
Laboratory of Clinical Science, National Institute of Mental Health, Poolesville, MD 20837.
Exp Brain Res. 1988;72(2):399-406. doi: 10.1007/BF00250261.
Fully mature rats were injected intraperitoneally with 3H-TdR on postnatal day (P) 100. After an additional 28-32 days, a retrograde fluorescent tracer, either FB or DY, was injected into the regio inferior of the hippocampal formation to label granule cells of the dentate gyrus through their mossy fiber axons. Examination of autoradiographs from these brains reveals that 3H-TdR labeled cells within the granule cell layer of the dentate gyrus are often labeled with the retrograde tracer as well. This indicates that within the mature hippocampal formation, newly generated dentate granule cells are capable of extending axonal projections for considerable distances.
在出生后第100天（P100），给完全成熟的大鼠腹腔注射3H-胸腺嘧啶核苷。再过28 - 32天后，将逆行荧光示踪剂FB或DY注入海马结构的下区，通过苔藓纤维轴突标记齿状回的颗粒细胞。对这些大脑的放射自显影片进行检查发现，齿状回颗粒细胞层内被3H-胸腺嘧啶核苷标记的细胞通常也被逆行示踪剂标记。这表明在成熟的海马结构中，新生成的齿状颗粒细胞能够将轴突投射延伸相当长的距离。
