Kaplan M S, Bell D H
J Neurosci. 1984 Jun;4(6):1429-41. doi: 10.1523/JNEUROSCI.04-06-01429.1984.
Ultrastructural identification of mitotic neuronal precursors beneath the basal hippocampal granule cell layer was made using electron micrographs of [3H]thymidine-labeled cells. Ultrathin sections were obtained by a method that allows serial thin sectioning of reembedded sections previously prepared for light microscopic radioautography. The electron microscopic observations reported in this study reveal: (1) that a steady rate of granule cell neurogenesis occurs during the first year of a rodent's life; (2) that newly formed granule neurons in the dentate gyrus of the newborn mouse and adult rat are a result of neuroblast division; and (3) two distinct classes of mitotic cells can be identified during the peak period of postnatal neurogenesis--those with synapses on their cell bodies and processes and those with no synapses or processes.
利用[3H]胸腺嘧啶核苷标记细胞的电子显微照片，对海马基底颗粒细胞层下方有丝分裂的神经元前体细胞进行了超微结构鉴定。超薄切片通过一种方法获得，该方法允许对先前为光学显微镜放射自显影制备的重新包埋切片进行连续薄切片。本研究报告的电子显微镜观察结果显示：（1）啮齿动物生命的第一年期间，颗粒细胞神经发生的速率稳定；（2）新生小鼠和成年大鼠齿状回中新生的颗粒神经元是神经母细胞分裂的结果；（3）在出生后神经发生的高峰期，可以识别出两类不同的有丝分裂细胞——那些在其细胞体和突起上有突触的细胞，以及那些没有突触或突起的细胞。
